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Title: Alterations in lipid composition and fluidity of liver plasma membranes in copper-deficient rats

Abstract

In view of the importance of membrane fluidity on cell functions, the influence of phospholipid acyl groups on membrane fluidity, and the changes in lipid metabolism induced by copper (Cu) deficiency, this study was designed to examine the influence of dietary Cu on the lipid composition and fluidity of liver plasma membranes. Male Sprague-Dawley rats were divided into two dietary treatments, namely Cu deficient and Cu adequate. After 8 weeks of treatment, liver plasma membranes were isolated by sucrose density gradient centrifugation. The lipid fluidity of plasma membranes, as assessed by the intramolecular eximer fluorescence of 1,3-di(1-pyrenyl) propane, was significantly depressed by Cu deficiency. In addition, Cu deficiency significantly reduced the content of arachidonic and palmitoleic acids but increased the docosatetraenoic acids of membrane phospholipids. This alteration in unsaturated phospholipid fatty acid composition, especially the large reduction in arachidonic acid, may have contributed to the depressed membrane fluidity. Furthermore, Cu deficiency also markedly altered the fatty acid composition of the triacylglycerols associated with the plasma membranes. Thus, the lipid composition and fluidity of liver plasma membranes are responsive to the animal's Cu status.

Authors:
; ; ; ; ;  [1]
  1. Univ. of Arizona, Tucson (USA)
Publication Date:
OSTI Identifier:
5026176
Resource Type:
Journal Article
Journal Name:
Proceedings of the Society for Experimental Biology and Medicine; (USA)
Additional Journal Information:
Journal Volume: 188:3; Journal ID: ISSN 0037-9727
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CELL MEMBRANES; ULTRASTRUCTURAL CHANGES; COPPER; BIOLOGICAL EFFECTS; LIPIDS; NUTRITIONAL DEFICIENCY; RATS; ANIMALS; CELL CONSTITUENTS; ELEMENTS; MAMMALS; MEMBRANES; METALS; MORPHOLOGICAL CHANGES; ORGANIC COMPOUNDS; RODENTS; TRANSITION ELEMENTS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Lei, K Y, Rosenstein, F, Shi, F, Hassel, C A, Carr, T P, and Zhang, J. Alterations in lipid composition and fluidity of liver plasma membranes in copper-deficient rats. United States: N. p., 1988. Web. doi:10.3181/00379727-188-42743.
Lei, K Y, Rosenstein, F, Shi, F, Hassel, C A, Carr, T P, & Zhang, J. Alterations in lipid composition and fluidity of liver plasma membranes in copper-deficient rats. United States. https://doi.org/10.3181/00379727-188-42743
Lei, K Y, Rosenstein, F, Shi, F, Hassel, C A, Carr, T P, and Zhang, J. 1988. "Alterations in lipid composition and fluidity of liver plasma membranes in copper-deficient rats". United States. https://doi.org/10.3181/00379727-188-42743.
@article{osti_5026176,
title = {Alterations in lipid composition and fluidity of liver plasma membranes in copper-deficient rats},
author = {Lei, K Y and Rosenstein, F and Shi, F and Hassel, C A and Carr, T P and Zhang, J},
abstractNote = {In view of the importance of membrane fluidity on cell functions, the influence of phospholipid acyl groups on membrane fluidity, and the changes in lipid metabolism induced by copper (Cu) deficiency, this study was designed to examine the influence of dietary Cu on the lipid composition and fluidity of liver plasma membranes. Male Sprague-Dawley rats were divided into two dietary treatments, namely Cu deficient and Cu adequate. After 8 weeks of treatment, liver plasma membranes were isolated by sucrose density gradient centrifugation. The lipid fluidity of plasma membranes, as assessed by the intramolecular eximer fluorescence of 1,3-di(1-pyrenyl) propane, was significantly depressed by Cu deficiency. In addition, Cu deficiency significantly reduced the content of arachidonic and palmitoleic acids but increased the docosatetraenoic acids of membrane phospholipids. This alteration in unsaturated phospholipid fatty acid composition, especially the large reduction in arachidonic acid, may have contributed to the depressed membrane fluidity. Furthermore, Cu deficiency also markedly altered the fatty acid composition of the triacylglycerols associated with the plasma membranes. Thus, the lipid composition and fluidity of liver plasma membranes are responsive to the animal's Cu status.},
doi = {10.3181/00379727-188-42743},
url = {https://www.osti.gov/biblio/5026176}, journal = {Proceedings of the Society for Experimental Biology and Medicine; (USA)},
issn = {0037-9727},
number = ,
volume = 188:3,
place = {United States},
year = {Fri Jul 01 00:00:00 EDT 1988},
month = {Fri Jul 01 00:00:00 EDT 1988}
}