Hyaluronate synthesis by synovial villi in organ culture. [Dogs]

Journal Article · Wed Jun 01 00:00:00 EDT 1983 · Arthritis Rheum.; (United States)

DOI:https://doi.org/10.1002/art.1780260610· OSTI ID:5024563

Myers, S L; Christine, T A

Individual canine synovial villi were used to establish short-term synovial organ cultures. These villi incorporated /sup 3/H-glucosamine into highly-polymerized /sup 3/H-hyaluronic acid (/sup 3/H-HA), which was the only /sup 3/H-glycosaminoglycan identified in the culture medium. Some /sup 3/H-HA, and larger amounts of other /sup 3/H-glycosaminoglycans, were recovered from cultured tissues. Culture medium /sup 3/H-HA content was proportional to the surface area of cultured villi. Organ cultures of nonvillous synovium were compared with villi; nonvillous cultures synthesized less /sup 3/H-HA per mm2 of their synovial intimal surface than villi. These cultures complement cell culture techniques for in vitro studies of synovial lining cell function.

• Research Organization: Indiana Univ. School of Medicine, Indianapolis
• OSTI ID: 5024563
• Journal Information: Arthritis Rheum.; (United States), Vol. 26:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE JOINTS
BIOCHEMISTRY
HYALURONIC ACID
BIOSYNTHESIS
DOGS
GLUCOSAMINE
TISSUE CULTURES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
BODY
CARBOHYDRATES
CHEMISTRY
HEXOSAMINES
HEXOSES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOSACCHARIDES
MUCOPOLYSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
SACCHARIDES
SKELETON
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques