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Title: Rubber transferase in guayule plants. [Parthenium argentatum]

Abstract

Rubber transferase catalyzes the transfer of cis-1,4-polyprenyl-PP to isopentenyl-PP (IPP) with the elimination of PP/sub i/. Rubber transferase activity in guayule (Parthenium argentatum Gray) stems was localized in the lipid fraction of the homogenate following centrifugation in buffer and 0.4M Mannitol. Washed rubber particles were obtained by the chromatography of the lipid fraction on Ultrogel columns with an exclusion limit of 750,000 daltons by the procedure of B.G. Audley (private communication). The rubber particles catalyzed the incorporation of /sup 14/C-IPP into cis-polyisoprene. The radioactive cis-polyisoprene was identified by ozonolysis and chromatography of the resulting /sup 14/C-levulinic acid. The synthesis of cis-polyisoprene in the rubber particles required Mg/sup 2 +/ and IPP and was stimulated 2-fold with the addition of DMAPP. Rubber synthesis in guayule plants growing in the Permian Basin of West Texas does not occur during summer months but is induced by the cold night temperatures of the fall and winter. From August to December individual plants (which were transplanted in May) accumulated from 66mg to 11,800mg or rubber. During this period there was a 4-fold increase in rubber transferase activity in stem homogenates induced by the low temperatures.

Authors:
; ;
Publication Date:
Research Org.:
Texas A and M Univ., College Station
OSTI Identifier:
5021752
Resource Type:
Conference
Journal Name:
Plant Physiol., Suppl.; (United States)
Additional Journal Information:
Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GUAYULE; TRANSFERASES; NATURAL RUBBER; BIOSYNTHESIS; AMBIENT TEMPERATURE; CARBON 14 COMPOUNDS; ENZYME ACTIVITY; LABELLING; ELASTOMERS; ENZYMES; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC POLYMERS; PLANTS; POLYMERS; RUBBER TREES; RUBBERS; SYNTHESIS; TREES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Rosenfield, C L, Foster, M A, and Benedict, C R. Rubber transferase in guayule plants. [Parthenium argentatum]. United States: N. p., 1986. Web.
Rosenfield, C L, Foster, M A, & Benedict, C R. Rubber transferase in guayule plants. [Parthenium argentatum]. United States.
Rosenfield, C L, Foster, M A, and Benedict, C R. Tue . "Rubber transferase in guayule plants. [Parthenium argentatum]". United States.
@article{osti_5021752,
title = {Rubber transferase in guayule plants. [Parthenium argentatum]},
author = {Rosenfield, C L and Foster, M A and Benedict, C R},
abstractNote = {Rubber transferase catalyzes the transfer of cis-1,4-polyprenyl-PP to isopentenyl-PP (IPP) with the elimination of PP/sub i/. Rubber transferase activity in guayule (Parthenium argentatum Gray) stems was localized in the lipid fraction of the homogenate following centrifugation in buffer and 0.4M Mannitol. Washed rubber particles were obtained by the chromatography of the lipid fraction on Ultrogel columns with an exclusion limit of 750,000 daltons by the procedure of B.G. Audley (private communication). The rubber particles catalyzed the incorporation of /sup 14/C-IPP into cis-polyisoprene. The radioactive cis-polyisoprene was identified by ozonolysis and chromatography of the resulting /sup 14/C-levulinic acid. The synthesis of cis-polyisoprene in the rubber particles required Mg/sup 2 +/ and IPP and was stimulated 2-fold with the addition of DMAPP. Rubber synthesis in guayule plants growing in the Permian Basin of West Texas does not occur during summer months but is induced by the cold night temperatures of the fall and winter. From August to December individual plants (which were transplanted in May) accumulated from 66mg to 11,800mg or rubber. During this period there was a 4-fold increase in rubber transferase activity in stem homogenates induced by the low temperatures.},
doi = {},
journal = {Plant Physiol., Suppl.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = {1986},
month = {4}
}

Conference:
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