Frequent mutation of the p53 gene in human esophageal cancer

Journal Article · Sat Dec 01 00:00:00 EST 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.24.9958· OSTI ID:5016935

Hollstein, M C; Montesano, R ^[1]; Metcalf, R A; Welsh, J A; Harris, C C ^[2]

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1. International Agency for Research on Cancer, Lyon (France)
2. National Institutes of Health, Bethesda, MD (United States)

Sequence alterations in the p53 gene have been detected in human tumors of the brain, breast, lung, and colon, and it has been proposed that p53 mutations spanning a major portion of the coding region inactivate the tumor suppressor function of this gene. To our knowledge, neither transforming mutations in oncogenes nor mutations in tumor suppressor genes have been reported in human esophageal tumors. The authors examined four human esophageal carcinoma cell lines and 14 human esophageal squamous cell carcinomas by polymerase chain reaction amplification and direct sequencing for the presence of p53 mutations in exons 5,6,7,8, and 9. Two cell lines and five of the tumor speicmens contained a mutated allele (one frameshift and six missense mutations). All missense mutations detected occurred at G{center dot}C base pairs in codons at or adjacent to mutations previously reported in other cancers. The identification of aberrant p53 genes alleles in one-third of the tumors they tested suggests that mutations at this locus are common genetic events in the pathogenesis of squamous cell carcinomas of the esophagus.

• OSTI ID: 5016935
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:24; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARCINOMAS
PATHOGENESIS
GENE MUTATIONS
DNA SEQUENCING
ONCOGENES
GENE REGULATION
BRAIN
ESOPHAGUS
LARGE INTESTINE
LUNGS
MAMMARY GLANDS
PHOSPHORUS 32
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
GASTROINTESTINAL TRACT
GENES
GLANDS
INTESTINES
ISOTOPES
LIGHT NUCLEI
MUTATIONS
NEOPLASMS
NERVOUS SYSTEM
NUCLEI
ODD-ODD NUCLEI
ORGANS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
RESPIRATORY SYSTEM
STRUCTURAL CHEMICAL ANALYSIS
550201* - Biochemistry- Tracer Techniques