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Title: Analysis of differential protein expression in normal and neoplastic human breast epithelial cell lines

Abstract

High resolution two dimensional get electrophoresis (2DE) and database analysis was used to establish protein expression patterns for cultured normal human mammary epithelial cells and thirteen breast cancer cell lines. The Human Breast Epithelial Cell database contains the 2DE protein patterns, including relative protein abundances, for each cell line, plus a composite pattern that contains all the common and specifically expressed proteins from all the cell lines. Significant differences in protein expression, both qualitative and quantitative, were observed not only between normal cells and tumor cells, but also among the tumor cell lines. Eight percent of the consistently detected proteins were found in significantly (P < 0.001) variable levels among the cell lines. Using a combination of immunostaining, comigration with purified protein, subcellular fractionation, and amino-terminal protein sequencing, we identified a subset of the differentially expressed proteins. These identified proteins include the cytoskeletal proteins actin, tubulin, vimentin, and cytokeratins. The cell lines can be classified into four distinct groups based on their intermediate filament protein profile. We also identified heat shock proteins; hsp27, hsp60, and hsp70 varied in abundance and in some cases in the relative phosphorylation levels among the cell lines. Finally, we identified IMP dehydrogenase in each ofmore » the cell lines, and found the levels of this enzyme in the tumor cell lines elevated 2- to 20-fold relative to the levels in normal cells.« less

Authors:
; ; ;
Publication Date:
Research Org.:
Argonne National Lab., IL (United States)
Sponsoring Org.:
USDOE Office of Energy Research, Washington, DC (United States)
OSTI Identifier:
501554
Report Number(s):
ANL/CMB/PP-92026
ON: DE97007231; TRN: 97:004389
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; TUMOR CELLS; PROTEIN STRUCTURE; MAMMARY GLANDS; ANIMAL CELLS; ACTIN; ELECTROPHORESIS; FRACTIONATION; HEAT-SHOCK PROTEINS; PHOSPHORYLATION; WOMEN; OXIDOREDUCTASES; DIAGNOSTIC TECHNIQUES; COMPARATIVE EVALUATIONS; ENZYME ACTIVITY; STRUCTURE-ACTIVITY RELATIONSHIPS

Citation Formats

Williams, K., Chubb, C., Huberman, E., and Giometti, C.S.. Analysis of differential protein expression in normal and neoplastic human breast epithelial cell lines. United States: N. p., 1997. Web. doi:10.2172/501554.
Williams, K., Chubb, C., Huberman, E., & Giometti, C.S.. Analysis of differential protein expression in normal and neoplastic human breast epithelial cell lines. United States. doi:10.2172/501554.
Williams, K., Chubb, C., Huberman, E., and Giometti, C.S.. Tue . "Analysis of differential protein expression in normal and neoplastic human breast epithelial cell lines". United States. doi:10.2172/501554. https://www.osti.gov/servlets/purl/501554.
@article{osti_501554,
title = {Analysis of differential protein expression in normal and neoplastic human breast epithelial cell lines},
author = {Williams, K. and Chubb, C. and Huberman, E. and Giometti, C.S.},
abstractNote = {High resolution two dimensional get electrophoresis (2DE) and database analysis was used to establish protein expression patterns for cultured normal human mammary epithelial cells and thirteen breast cancer cell lines. The Human Breast Epithelial Cell database contains the 2DE protein patterns, including relative protein abundances, for each cell line, plus a composite pattern that contains all the common and specifically expressed proteins from all the cell lines. Significant differences in protein expression, both qualitative and quantitative, were observed not only between normal cells and tumor cells, but also among the tumor cell lines. Eight percent of the consistently detected proteins were found in significantly (P < 0.001) variable levels among the cell lines. Using a combination of immunostaining, comigration with purified protein, subcellular fractionation, and amino-terminal protein sequencing, we identified a subset of the differentially expressed proteins. These identified proteins include the cytoskeletal proteins actin, tubulin, vimentin, and cytokeratins. The cell lines can be classified into four distinct groups based on their intermediate filament protein profile. We also identified heat shock proteins; hsp27, hsp60, and hsp70 varied in abundance and in some cases in the relative phosphorylation levels among the cell lines. Finally, we identified IMP dehydrogenase in each of the cell lines, and found the levels of this enzyme in the tumor cell lines elevated 2- to 20-fold relative to the levels in normal cells.},
doi = {10.2172/501554},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 01 00:00:00 EDT 1997},
month = {Tue Jul 01 00:00:00 EDT 1997}
}

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