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Title: In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles

Abstract

The potent mutagen and environmental pollutant 1-nitropyrene (NP) labeled with /sup 14/C and vapour-phase-coated onto diesel particles (/sup 14/C-NP-DP; 380 ppm NP; 0.85 microCi/mg particles) was administered to rats (5 mg each) by oral or by intratracheal instillation. 6-Hydroxy-N-acetyl-1-aminopyrene was previously found to be the metabolite responsible for the majority of the mutagenic activity in the urines of rats injected with pure NP. Phenols of NP itself were also identified, both free and conjugated, representing 1-5% of the urinary /sup 14/C. The extent of urinary excretion indicated that NP was readily bioavailable from the dose remained in the lung 24 h after intratracheal instillation, and 5 to 15% of that /sup 14/C appeared to be bound to protein. Since NP has previously been shown to bind to lung DNA and protein in vitro, these results indicate that this organ might be particularly exposed in vivo and therefore vulnerable to possible genotoxic damage by NP.

Authors:
; ; ;
Publication Date:
Research Org.:
Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
OSTI Identifier:
5010895
Report Number(s):
PB-85-218857/XAB
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DIESEL FUELS; ADDITIVES; MUTAGENS; CHEMICAL BONDS; METABOLISM; AIR POLLUTION; CARBON 14 COMPOUNDS; LABORATORY ANIMALS; PARTICLES; PUBLIC HEALTH; TOXICITY; LABELLED COMPOUNDS; PETROLEUM PRODUCTS; POLLUTION; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Ball, L M, Jackson, M A, King, L C, and Lewtas, J. In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles. United States: N. p., 1985. Web.
Ball, L M, Jackson, M A, King, L C, & Lewtas, J. In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles. United States.
Ball, L M, Jackson, M A, King, L C, and Lewtas, J. Tue . "In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles". United States.
@article{osti_5010895,
title = {In vivo metabolism, disposition, and macromolecular binding of 1-nitro (/sup 14/C) pyrene vapor-coated onto diesel particles},
author = {Ball, L M and Jackson, M A and King, L C and Lewtas, J},
abstractNote = {The potent mutagen and environmental pollutant 1-nitropyrene (NP) labeled with /sup 14/C and vapour-phase-coated onto diesel particles (/sup 14/C-NP-DP; 380 ppm NP; 0.85 microCi/mg particles) was administered to rats (5 mg each) by oral or by intratracheal instillation. 6-Hydroxy-N-acetyl-1-aminopyrene was previously found to be the metabolite responsible for the majority of the mutagenic activity in the urines of rats injected with pure NP. Phenols of NP itself were also identified, both free and conjugated, representing 1-5% of the urinary /sup 14/C. The extent of urinary excretion indicated that NP was readily bioavailable from the dose remained in the lung 24 h after intratracheal instillation, and 5 to 15% of that /sup 14/C appeared to be bound to protein. Since NP has previously been shown to bind to lung DNA and protein in vitro, these results indicate that this organ might be particularly exposed in vivo and therefore vulnerable to possible genotoxic damage by NP.},
doi = {},
url = {https://www.osti.gov/biblio/5010895}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1985},
month = {1}
}

Technical Report:
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