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Title: Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells

Abstract

The effect of RSV infection on the adherence of Streptococcus pneumoniae (SP), Haemophilus influenzae (HI) and Staphylococcus aureus (SA) to human epithelial cells was determined. RSV-infected Hep-2 cell cultures at different stages of expression of surface viral antigens and bacteria labeled with /sup 3/H-thymidine were employed to examine the kinetics of bacterial adherence to virus-infected cells. RSV infection did not alter the magnitude of adherence of HI or SA to HEp-2 cells. However, adherence of SP to HEp-2 cells was significantly (P < 0.01) enhanced by prior RSV infection. The degree of adherence was directly related to the amount of viral antigen expressed on the cell surface. The adherence was temperature dependent, with maximal adherence observed at 37/sup 0/C. Heat-inactivation of SP did not alter adherence characteristics. These data suggest that RSV infection increases adherence of SP to the surface of epithelial cells in vitro. Since attachment of bacteria to mucosal surfaces is the first step in many infections, it is suggested that viral infections of epithelial cells render them more susceptible to bacterial adherence. Thus, RSV infection in vivo may predispose children to SP infections, such as in otitis media, by increasing colonization with SP.

Authors:
; ;
Publication Date:
Research Org.:
State Univ. of New York, Buffalo
OSTI Identifier:
5006886
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-037028
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIAL DISEASES; PATHOGENESIS; HAEMOPHILUS; ADHESION; STAPHYLOCOCCUS; STREPTOCOCCUS; VIRAL DISEASES; BIOLOGICAL EFFECTS; EPITHELIUM; INFECTIVITY; PATHOGENS; RESPIRATORY SYSTEM; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL TISSUES; AZINES; BACTERIA; BODY; DISEASES; HETEROCYCLIC COMPOUNDS; INFECTIOUS DISEASES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MICROORGANISMS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRIMIDINES; RIBOSIDES; TISSUES; 550901* - Pathology- Tracer Techniques

Citation Formats

Faden, H., Hong, J.J., and Ogra, P.L. Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells. United States: N. p., 1986. Web.
Faden, H., Hong, J.J., & Ogra, P.L. Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells. United States.
Faden, H., Hong, J.J., and Ogra, P.L. Sat . "Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells". United States.
@article{osti_5006886,
title = {Effect of respiratory syncytial virus (RSV) infection on the adherence of pathogenic bacteria to human epithelial cells},
author = {Faden, H. and Hong, J.J. and Ogra, P.L.},
abstractNote = {The effect of RSV infection on the adherence of Streptococcus pneumoniae (SP), Haemophilus influenzae (HI) and Staphylococcus aureus (SA) to human epithelial cells was determined. RSV-infected Hep-2 cell cultures at different stages of expression of surface viral antigens and bacteria labeled with /sup 3/H-thymidine were employed to examine the kinetics of bacterial adherence to virus-infected cells. RSV infection did not alter the magnitude of adherence of HI or SA to HEp-2 cells. However, adherence of SP to HEp-2 cells was significantly (P < 0.01) enhanced by prior RSV infection. The degree of adherence was directly related to the amount of viral antigen expressed on the cell surface. The adherence was temperature dependent, with maximal adherence observed at 37/sup 0/C. Heat-inactivation of SP did not alter adherence characteristics. These data suggest that RSV infection increases adherence of SP to the surface of epithelial cells in vitro. Since attachment of bacteria to mucosal surfaces is the first step in many infections, it is suggested that viral infections of epithelial cells render them more susceptible to bacterial adherence. Thus, RSV infection in vivo may predispose children to SP infections, such as in otitis media, by increasing colonization with SP.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {1986},
month = {3}
}

Conference:
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