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Title: (Basis for the competitiveness of Rhizobium japonicum in nodulation of soybean). Progress report, 1984

Technical Report ·
OSTI ID:5006040

Those characteristics of Rhizobium cells that are most crucial in determining their competitive success when inoculated onto seed in the field are sought. Initial studies of Rhizobium attachment to root surfaces revealed that only a small subpopulation of the cells in an R. japonicum culture are capable of firmly attaching to soybean roots. The size of the attachment-competent subpopulation depends on strain and culture age. Attachment of rhizobia to roots was found to be linearly proportional to the bacterial concentration. The rate of attachment is constant under our conditions for approximately 60 min, then rapidly levels off to approximately zero. Once attached to the root surface, Rhizobium cells seldom spontaneously detach. Rhizobia of several different species all attached comparably well to soybean roots. Attachment of various Rhizobium species to the root hairs of soybean seedlings likewise showed no evidence of host specificity or selectivity. 2 figs., 2 tabs.

Research Organization:
Battelle-Charles F. Kettering Research Lab., Yellow Springs, OH (USA)
DOE Contract Number:
FG02-84ER13211
OSTI ID:
5006040
Report Number(s):
DOE/ER/13211-1; ON: DE87002014
Resource Relation:
Other Information: Portions of this document are illegible in microfiche products
Country of Publication:
United States
Language:
English