skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice

Abstract

The effects of inhaled benzene on primary and secondary antibody responses and the incidence of leukemia in mice are reported. Young adult mice were given 5, 12, or 22 exposures to 400 ppM benzene for 6 hrs/day 5 days/week. After the exposure periods, the mice were immunized with absorbed tetanus toxoid (APTT) and/or fluid tetanus toxid (FTT). Exposure to benzene increasingly suppressed primary antibody responses to both antigens. Secondary antibody responses to FTT were nearly normal in animals given 10, 15, or 20 exposures to 400 ppM benzene. Other groups of mice were exposed to either 200 ppM or 50 ppM benzene. Primary antibody responses elicited with FTT and/or APTT were nearly normal in all mice exposed to 50 ppM benzene and in mice exposed to 200 ppM benzene for 5 days. However, 10 and 20 exposures to 200 ppM benzene inhibited antibody production. The effects of chronically inhaled 300 ppM benzene on the time of onset and incidence of leukemia in 400 7-month-old female HRS/J mice were also studied. Two genotypes were used; the (hr/hr) hairless mice are leukemia-prone, whereas the (hr/+) haired mice are more resistant to leukemia. The exposure continued for a period of 6 months. Lymphoid,more » myeloid, and mixed (lymphoid and myeloid) leukemias were observed. Ninety percent of the (hr/hr) mice exposed to benzene died from leukemia as compared with 91% for the (hr/hr) air control group. Eighty-five percent of the (hr/+) mice exposed to benzene died from leukemia as compared with 81% for the (hr/+) air control group. Exposures to 300 ppM benzene did not alter the time of onset or the incidence of leukemia commonly expected in HRS/J mice.« less

Authors:
; ;
Publication Date:
Research Org.:
Brookhaven National Lab., Upton, NY (USA)
OSTI Identifier:
5004543
Report Number(s):
BNL-28428; CONF-801039-2
DOE Contract Number:  
AC02-76CH00016
Resource Type:
Conference
Resource Relation:
Conference: 20. annual Hanford Life Sciences symposium, Richland, WA, USA, 19 Oct 1980
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BENZENE; BIOLOGICAL EFFECTS; LEUKEMOGENESIS; IMMUNE REACTIONS; DOSE-RESPONSE RELATIONSHIPS; IMMUNITY; PATHOLOGICAL CHANGES; LEUKEMIA; GENETIC VARIABILITY; MICE; SENSITIVITY; ADULTS; ANTIBODY FORMATION; CHRONIC EXPOSURE; EXPERIMENTAL DATA; EXPOSURE CHAMBERS; FEMALES; GENOTYPE; INHALATION; TETANUS; TOXICITY; TOXINS; AGE GROUPS; ANIMALS; ANTIGENS; AROMATICS; BIOLOGICAL VARIABILITY; CARCINOGENESIS; DATA; DISEASES; HEMIC DISEASES; HYDROCARBONS; INFECTIOUS DISEASES; INFORMATION; INTAKE; MAMMALS; MATERIALS; NEOPLASMS; NUMERICAL DATA; ORGANIC COMPOUNDS; PATHOGENESIS; RODENTS; TOXIC MATERIALS; VERTEBRATES; 560306* - Chemicals Metabolism & Toxicology- Man- (-1987); 551000 - Physiological Systems; 550900 - Pathology

Citation Formats

Stoner, R D, Drew, R T, and Bernstein, D M. Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice. United States: N. p., 1980. Web.
Stoner, R D, Drew, R T, & Bernstein, D M. Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice. United States.
Stoner, R D, Drew, R T, and Bernstein, D M. Tue . "Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice". United States. https://www.osti.gov/servlets/purl/5004543.
@article{osti_5004543,
title = {Benzene inhalation effects upon tetanus antitoxin. Responses and leukemogenesis in mice},
author = {Stoner, R D and Drew, R T and Bernstein, D M},
abstractNote = {The effects of inhaled benzene on primary and secondary antibody responses and the incidence of leukemia in mice are reported. Young adult mice were given 5, 12, or 22 exposures to 400 ppM benzene for 6 hrs/day 5 days/week. After the exposure periods, the mice were immunized with absorbed tetanus toxoid (APTT) and/or fluid tetanus toxid (FTT). Exposure to benzene increasingly suppressed primary antibody responses to both antigens. Secondary antibody responses to FTT were nearly normal in animals given 10, 15, or 20 exposures to 400 ppM benzene. Other groups of mice were exposed to either 200 ppM or 50 ppM benzene. Primary antibody responses elicited with FTT and/or APTT were nearly normal in all mice exposed to 50 ppM benzene and in mice exposed to 200 ppM benzene for 5 days. However, 10 and 20 exposures to 200 ppM benzene inhibited antibody production. The effects of chronically inhaled 300 ppM benzene on the time of onset and incidence of leukemia in 400 7-month-old female HRS/J mice were also studied. Two genotypes were used; the (hr/hr) hairless mice are leukemia-prone, whereas the (hr/+) haired mice are more resistant to leukemia. The exposure continued for a period of 6 months. Lymphoid, myeloid, and mixed (lymphoid and myeloid) leukemias were observed. Ninety percent of the (hr/hr) mice exposed to benzene died from leukemia as compared with 91% for the (hr/hr) air control group. Eighty-five percent of the (hr/+) mice exposed to benzene died from leukemia as compared with 81% for the (hr/+) air control group. Exposures to 300 ppM benzene did not alter the time of onset or the incidence of leukemia commonly expected in HRS/J mice.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1980},
month = {1}
}

Conference:
Other availability
Please see Document Availability for additional information on obtaining the full-text document. Library patrons may search WorldCat to identify libraries that hold this conference proceeding.

Save / Share: