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Title: Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice

Abstract

Different groups of C57BL/ka or BALB/c mice received a dose of 34 Gy or 42 Gy of fractionated total lymphoid irradiation (TLI) before bone marrow transplantation with 30 x 10/sup 6/ BALB/c or C57BL nucleated bone marrow cells, respectively. BALB/c mice that were not bred in specific pathogen-free conditions before TLI showed a high morbidity and mortality rate after 34 Gy of TLI and allogeneic bone marrow transplantation as compared with BALB/c or C57BL that were bred in pathogen-free conditions before irradiation. Many of the conventionally bred BALB/c mice had clinical and histologic signs of graft-vs-host disease after TLI and allogeneic bone marrow infusion. Although leucocytosis and lymphocytosis and the immunologic competence as measured with in vitro tests were equally depressed after 34 Gy TLI in BALB/c and C57BL mice, chimerism was nevertheless significantly easier to obtain in BALB/c mice. The incidence of chimerism after TLI could be enhanced in C57BL mice by increasing the total radiation dose from 34 to 42 Gy. This augmentation of chimerism was paralleled by the induction of more suppressor cells after 42 Gy of TLI in C57BL mice.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of Leuven, Belgium
OSTI Identifier:
5004001
Resource Type:
Journal Article
Journal Name:
J. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 132:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANIMAL BREEDING; EVALUATION; GENETIC VARIABILITY; IMMUNOSUPPRESSION; RADIOINDUCTION; MICE; BIOLOGICAL RADIATION EFFECTS; BONE MARROW; COBALT 60; FRACTIONATED IRRADIATION; GAMMA RADIATION; LYMPHATIC SYSTEM; LYMPHOCYTES; PARTIAL BODY IRRADIATION; RADIATION CHIMERAS; TRANSPLANTS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL VARIABILITY; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHIMERAS; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; MOSAICISM; NUCLEI; ODD-ODD NUCLEI; ORGANS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Waer, M., Ang, K.K., van der Schueren, E., and Vandeputte, M. Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice. United States: N. p., 1984. Web.
Waer, M., Ang, K.K., van der Schueren, E., & Vandeputte, M. Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice. United States.
Waer, M., Ang, K.K., van der Schueren, E., and Vandeputte, M. Wed . "Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice". United States.
@article{osti_5004001,
title = {Allogeneic bone marrow transplantation in mice after total lymphoid irradiation: influence of breeding conditions and strain of recipient mice},
author = {Waer, M. and Ang, K.K. and van der Schueren, E. and Vandeputte, M.},
abstractNote = {Different groups of C57BL/ka or BALB/c mice received a dose of 34 Gy or 42 Gy of fractionated total lymphoid irradiation (TLI) before bone marrow transplantation with 30 x 10/sup 6/ BALB/c or C57BL nucleated bone marrow cells, respectively. BALB/c mice that were not bred in specific pathogen-free conditions before TLI showed a high morbidity and mortality rate after 34 Gy of TLI and allogeneic bone marrow transplantation as compared with BALB/c or C57BL that were bred in pathogen-free conditions before irradiation. Many of the conventionally bred BALB/c mice had clinical and histologic signs of graft-vs-host disease after TLI and allogeneic bone marrow infusion. Although leucocytosis and lymphocytosis and the immunologic competence as measured with in vitro tests were equally depressed after 34 Gy TLI in BALB/c and C57BL mice, chimerism was nevertheless significantly easier to obtain in BALB/c mice. The incidence of chimerism after TLI could be enhanced in C57BL mice by increasing the total radiation dose from 34 to 42 Gy. This augmentation of chimerism was paralleled by the induction of more suppressor cells after 42 Gy of TLI in C57BL mice.},
doi = {},
journal = {J. Immunol.; (United States)},
number = ,
volume = 132:2,
place = {United States},
year = {1984},
month = {2}
}