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Title: Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation

Abstract

Performance data on slurry-phase bioremediation of pentachlorophenol- (PCP-) and creosote-contaminated sediment and surface soil were generated at the bench-scale level. Aqueous slurries were prepared from sediment and surface soil freshly obtained from the American Creosote Works Superfund site at Pensacola, FL. Slurries (1.1 L) were incubated for 30 days in separate, 1.5-L bioreactors operated in the batch mode at 28.5C with continuous mixing (300 rpm), DO = 90% and pH = 7.0. Samples removed with time from each reactor were extracted and analyzed by gas chromatography for PCP and 42 monitored creosote constituents to delineate the activity of indigenous microorganisms. Changes in microbial biomass were also recorded. Excluding PCP, benzo(b)fluoranthene, benzo(k)fluoranthene, and indeno(1,2,3-cd)pyrene, slurry-phase bioremediation of highly contaminated sediment (pH adjusted) resulted in rapid and extensive biodegradation (3-5 days to biodegrade > 50% of targeted compounds) of monitored constituents. Conversely, microbial activity in surface soil slurries was slower and generally confined to the more readily biodegradable, lower molecular weight compounds. These data suggest that slurry-phase bioremediation strategies can be effectively employed to remediate creosote-contaminated materials.

Authors:
;  [1];  [2];  [3]
  1. Southern Bio Products, Inc., Gulf Breeze, Fl (United States)
  2. Technical Resources, Inc., Gulf Breeze, FL (United States)
  3. Environmental Protection Agency, Gulf Breeze, FL (United States)
Publication Date:
OSTI Identifier:
5003698
Resource Type:
Journal Article
Journal Name:
Environmental Science and Technology; (United States)
Additional Journal Information:
Journal Volume: 25:6; Journal ID: ISSN 0013-936X
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; CHLORINATED AROMATIC HYDROCARBONS; BIODEGRADATION; CREOSOTE; SOILS; DECONTAMINATION; BENCH-SCALE EXPERIMENTS; BIOMASS; COMPARATIVE EVALUATIONS; DERIVATIZATION; DIAGRAMS; FERTILIZERS; GAS CHROMATOGRAPHY; GROUND WATER; MICROORGANISMS; POLLUTANTS; POLYCYCLIC AROMATIC HYDROCARBONS; PROTEINS; QUALITY ASSURANCE; QUALITY CONTROL; QUANTITATIVE CHEMICAL ANALYSIS; REMEDIAL ACTION; SEDIMENTS; SILANES; SLURRIES; SOLVENT EXTRACTION; US SUPERFUND; AROMATICS; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; CHROMATOGRAPHY; CLEANING; CONTROL; DECOMPOSITION; DISPERSIONS; ENERGY SOURCES; EVALUATION; EXTRACTION; HALOGENATED AROMATIC HYDROCARBONS; HYDRIDES; HYDROCARBONS; HYDROGEN COMPOUNDS; LAWS; MIXTURES; ORGANIC CHLORINE COMPOUNDS; ORGANIC COMPOUNDS; ORGANIC HALOGEN COMPOUNDS; ORGANIC SILICON COMPOUNDS; OXYGEN COMPOUNDS; POLLUTION LAWS; RENEWABLE ENERGY SOURCES; SEPARATION PROCESSES; SILICON COMPOUNDS; SUSPENSIONS; WATER; 540220* - Environment, Terrestrial- Chemicals Monitoring & Transport- (1990-); 400102 - Chemical & Spectral Procedures

Citation Formats

Mueller, J G, Lantz, S E, Blattmann, B O, and Chapman, P J. Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation. United States: N. p., 1991. Web. doi:10.1021/es00018a006.
Mueller, J G, Lantz, S E, Blattmann, B O, & Chapman, P J. Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation. United States. https://doi.org/10.1021/es00018a006
Mueller, J G, Lantz, S E, Blattmann, B O, and Chapman, P J. Sat . "Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation". United States. https://doi.org/10.1021/es00018a006.
@article{osti_5003698,
title = {Bench-scale evaluation of alternative biological treatment processes for the remediation of pentachlorophenol- and creosote-contaminated materials: Slurry-phase bioremediation},
author = {Mueller, J G and Lantz, S E and Blattmann, B O and Chapman, P J},
abstractNote = {Performance data on slurry-phase bioremediation of pentachlorophenol- (PCP-) and creosote-contaminated sediment and surface soil were generated at the bench-scale level. Aqueous slurries were prepared from sediment and surface soil freshly obtained from the American Creosote Works Superfund site at Pensacola, FL. Slurries (1.1 L) were incubated for 30 days in separate, 1.5-L bioreactors operated in the batch mode at 28.5C with continuous mixing (300 rpm), DO = 90% and pH = 7.0. Samples removed with time from each reactor were extracted and analyzed by gas chromatography for PCP and 42 monitored creosote constituents to delineate the activity of indigenous microorganisms. Changes in microbial biomass were also recorded. Excluding PCP, benzo(b)fluoranthene, benzo(k)fluoranthene, and indeno(1,2,3-cd)pyrene, slurry-phase bioremediation of highly contaminated sediment (pH adjusted) resulted in rapid and extensive biodegradation (3-5 days to biodegrade > 50% of targeted compounds) of monitored constituents. Conversely, microbial activity in surface soil slurries was slower and generally confined to the more readily biodegradable, lower molecular weight compounds. These data suggest that slurry-phase bioremediation strategies can be effectively employed to remediate creosote-contaminated materials.},
doi = {10.1021/es00018a006},
url = {https://www.osti.gov/biblio/5003698}, journal = {Environmental Science and Technology; (United States)},
issn = {0013-936X},
number = ,
volume = 25:6,
place = {United States},
year = {1991},
month = {6}
}