Immunoassay directed field screening simplifies site assessment of PAH in soil and water
- Strategic Diagnostics Inc., Newark, DE (United States)
Until recently, site assessment of PAH levels was limited by the high cost and long turnaround time associated with the analytical laboratory techniques for measuring PAH. An immunoassay for the semi-quantitative detection of PAH was developed as a part of the D TECH{trademark} environmental detection systems line of products. This PAH immunoassay is designed to simplify the process of site assessment by providing a rapid, reliable, and low cost alternative to analytical laboratory techniques. In the immunoassay, PAH in a water sample or extract from a soil sample inhibits the binding of a latex-immobilized anti-PAH antibody to an alkaline phosphatase labeled PAH analog. This test detects the majority of the compounds on the EPA`s list of 16 PAH priority pollutants and its specificity is directed toward the 3, 4 and 5 ring compounds. The assay detects the carcinogen benzo(a)pyrene and does not detect BTEX, PCB, or other priority pollutants. Contaminated field samples of PAH were collected and the PAH concentrations were determined by both the immunoassay and a commercial laboratory using the EPA SW-846 method 8270. The immunoassay results correlated with method 8270 and displayed sensitivity below the ppm level in soil and within the ppb range for water. The low number of false positive and false negative results confirmed the specificity of the immunoassay for PAHs.
- OSTI ID:
- 49400
- Report Number(s):
- CONF-941189-; ISBN 1-56590-016-2; TRN: IM9523%%223
- Resource Relation:
- Conference: SUPERFUND XV: 15th environmental conference and exhibition for the hazardous materials/hazardous waste management industry, Washington, DC (United States), 29 Nov - 1 Dec 1994; Other Information: PBD: 1994; Related Information: Is Part Of Superfund XV conference proceedings. Volume 1; PB: 866 p.
- Country of Publication:
- United States
- Language:
- English
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