Biodegradation of PCP in soil
- National Environmental Research Inst., Roskilde (Denmark)
- A/S Bioteknisk Jordrens, Kalundborg (Denmark)
- Univ. of Helsinki (Finland). Dept. of Applied Chemistry and Microbiology
Survival and activity of the pentachlorophenol (PCP)-mineralizer Mycobacterium chlorophenolicum after inoculation into a natural soil were studied at bench and field scales. The PCP-concentration was 30 mg kg{sup {minus}1} soil. Inoculation levels were 10{sup 6} to 10{sup 9} cells g{sup {minus}1} soil. The effect of immobilization in the inocula on polyurethane foam (PUF) was investigated at bench scale. PCP-mineralization was measured using {sup 14}C-PCP at bench scale and by soil chemical analyses in the field. The population dynamics of the degrader were followed by immunofluorescence microscopy using specific immunoprobes. It was shown that M. chlorophenolicum was still present at 1 order of magnitude below inoculation level with both immobilized and nonimmobilized cells after up to 1 year. However, inoculation at 10{sup 8} and 10{sup 6} cells g{sup {minus}1} soil did not raise the PCP-mineralization rate over the background level when cells had not been immobilized. Inoculation with PUF-immobilized cells significantly stimulated the rate of biodegradation at bench and field scales. Formation of chloroanisols or chloroveratroles through biomethylation of chlorophenols was observed upon inoculation with nonimmobilized cells at field scale.
- OSTI ID:
- 490973
- Report Number(s):
- CONF-950483-; ISBN 1-57477-008-X; TRN: IM9729%%224
- Resource Relation:
- Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Bioremediation of recalcitrant organics; Hinchee, R.E.; Anderson, D.B. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Hoeppel, R.E. [ed.] [Naval Facilities Engineering Services Center, Port Hueneme, CA (United States)]; PB: 378 p.; Bioremediation, Volume 3(7)
- Country of Publication:
- United States
- Language:
- English
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