Detoxification of aromatic pollutants by fungal enzymes

Bollag, J M; Dec, J

Title: Detoxification of aromatic pollutants by fungal enzymes

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Abstract

Fungal enzymes, such as laccase, peroxidase, and tyrosinase, play a prominent role in catalyzing the transformation of various aromatic compounds in the environment. The enzyme-mediated oxidative coupling reaction results in covalent binding of chlorinated phenols and anilines to soil organic matter or polymerization of the substrates in aquatic systems. Both of these processes are accompanied by a detoxification effect. Therefore, it has been postulated that they be exploited for the treatment of polluted soil and water. The mechanism and efficiency of oxidative coupling in pollutant removal were studied by incubation of chlorinated phenols and anilines with various humic substances or soil and analysis of the reaction products by chromatography and mass and {sup 13}C nuclear magnetic resonance (NMR) spectrometry. The decontamination effect could be enhanced by optimization of the reaction conditions and immobilization of enzymes on solid materials. The results obtained strongly support the concept of using enzymes for control of environmental pollution.

Authors:

Bollag, J M; Dec, J ^[1]

Pennsylvania State Univ., University Park, PA (United States)

Publication Date:: Sun Dec 31 00:00:00 EST 1995

OSTI Identifier:: 484950

Report Number(s):: CONF-950483-
ISBN 1-57477-009-8; TRN: IM9727%%118

Resource Type:: Conference

Resource Relation:: Conference: 3. international in situ and on-site bioreclamation symposium, San Diego, CA (United States), 24-27 Apr 1995; Other Information: PBD: 1995; Related Information: Is Part Of Microbial processes for bioremediation; Hinchee, R.E.; Brockman, F.J. [eds.] [Battelle Memorial Inst., Columbus, OH (United States)]; Vogel, C.M. [ed.]; PB: 371 p.; Bioremediation, Volume 3(8)

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; BIODEGRADATION; CHLORINATED AROMATIC HYDROCARBONS; FUNGI; REMEDIAL ACTION; NMR SPECTRA; METABOLISM; ENZYME ACTIVITY; PEROXIDASES; TYROSINASE

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                    Bollag, J M, and Dec, J. Detoxification of aromatic pollutants by fungal enzymes.  United States: N. p., 1995. 
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                    Bollag, J M, & Dec, J. Detoxification of aromatic pollutants by fungal enzymes.  United States.

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                    Bollag, J M, and Dec, J. 1995.  
        "Detoxification of aromatic pollutants by fungal enzymes".  United States.

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@article{osti_484950,

  title        = {Detoxification of aromatic pollutants by fungal enzymes},

  author       = {Bollag, J M and Dec, J},

  abstractNote = {Fungal enzymes, such as laccase, peroxidase, and tyrosinase, play a prominent role in catalyzing the transformation of various aromatic compounds in the environment. The enzyme-mediated oxidative coupling reaction results in covalent binding of chlorinated phenols and anilines to soil organic matter or polymerization of the substrates in aquatic systems. Both of these processes are accompanied by a detoxification effect. Therefore, it has been postulated that they be exploited for the treatment of polluted soil and water. The mechanism and efficiency of oxidative coupling in pollutant removal were studied by incubation of chlorinated phenols and anilines with various humic substances or soil and analysis of the reaction products by chromatography and mass and {sup 13}C nuclear magnetic resonance (NMR) spectrometry. The decontamination effect could be enhanced by optimization of the reaction conditions and immobilization of enzymes on solid materials. The results obtained strongly support the concept of using enzymes for control of environmental pollution.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/484950},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun Dec 31 00:00:00 EST 1995},

  month        = {Sun Dec 31 00:00:00 EST 1995}

}

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