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Title: CHROMOSOME ABERRATIONS, CHANGES IN DNA CONTENT AND FREQUENCY AND SPECTRUM OF MUTATIONS INDUCED BY X-RAYS AND NEUTRONS IN POLYPLOIDS

Abstract

A study of the relation between polyploidy and radiosensitivity was carried out in diploid, tetraploid, and hexaploid species of wheat and in diploid and autotetraploid barley using x rays and fast and thermal neutruns. Survival, frequeney of chromosome aberrations, DNA content of nuclei, seed fertility, and frequency of mutations in the second and third generations were used as indexes of radiosenst-tivity. Survival was better in 4x barley in comparison with the diploid in x-ray treatments, while an increase in radiation resistance with an increase in chromosome number was evident in the wheat series with fast neutrons. Data on the frequency of chromosome aberrations and reduction in DNA content observed in root-tip nuclei indicated a possible interaction between polyploidy and the LET of the radiation employed. Seed fertility was less affected in polyploids than in diploids. The frequency of chlorophyll mutations decreased with increasing ploidy in both wheat and barley while the frequency of viable mutations registered the opposite trend in wheat. Recessive mutations could be detected in 4x barley only in the M/sub 3/ generation. The relative proportion of chlorophyll and morphological mutations observed in 4x barley during the M/sub 3/ generation was similar to that found in 6x wheatmore » in M/sub 2/ and M/sub 3/ generations. Though a high frequency of viable mutations was observed in 6x wheat, the spectrum of the induced variation was very narrow, thereby indicating that this species is a functional diploid only for a few loci. (auth)« less

Authors:
Publication Date:
Research Org.:
Indian Agricultural Research Inst., New Delhi
OSTI Identifier:
4800392
NSA Number:
NSA-16-012933
Resource Type:
Journal Article
Journal Name:
Radiation Botany
Additional Journal Information:
Journal Volume: Vol: 1; Other Information: Orig. Receipt Date: 31-DEC-62
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
BIOLOGY AND MEDICINE; CEREALS; CHLOROPHYLL; CHROMOSOMES; FAST NEUTRONS; LIFETIME; MUTATIONS; NUCLEIC ACIDS; PLANT CELLS; QUANTITY RATIO; RADIATION INJURIES; RADIOSENSITIVITY; SEEDS; SPECTRA; THERMAL NEUTRONS; X RADIATION

Citation Formats

Bhaskaran, S.f Swaminathan, M.S. CHROMOSOME ABERRATIONS, CHANGES IN DNA CONTENT AND FREQUENCY AND SPECTRUM OF MUTATIONS INDUCED BY X-RAYS AND NEUTRONS IN POLYPLOIDS. Country unknown/Code not available: N. p., 1962. Web.
Bhaskaran, S.f Swaminathan, M.S. CHROMOSOME ABERRATIONS, CHANGES IN DNA CONTENT AND FREQUENCY AND SPECTRUM OF MUTATIONS INDUCED BY X-RAYS AND NEUTRONS IN POLYPLOIDS. Country unknown/Code not available.
Bhaskaran, S.f Swaminathan, M.S. 1962. "CHROMOSOME ABERRATIONS, CHANGES IN DNA CONTENT AND FREQUENCY AND SPECTRUM OF MUTATIONS INDUCED BY X-RAYS AND NEUTRONS IN POLYPLOIDS". Country unknown/Code not available.
@article{osti_4800392,
title = {CHROMOSOME ABERRATIONS, CHANGES IN DNA CONTENT AND FREQUENCY AND SPECTRUM OF MUTATIONS INDUCED BY X-RAYS AND NEUTRONS IN POLYPLOIDS},
author = {Bhaskaran, S.f Swaminathan, M.S.},
abstractNote = {A study of the relation between polyploidy and radiosensitivity was carried out in diploid, tetraploid, and hexaploid species of wheat and in diploid and autotetraploid barley using x rays and fast and thermal neutruns. Survival, frequeney of chromosome aberrations, DNA content of nuclei, seed fertility, and frequency of mutations in the second and third generations were used as indexes of radiosenst-tivity. Survival was better in 4x barley in comparison with the diploid in x-ray treatments, while an increase in radiation resistance with an increase in chromosome number was evident in the wheat series with fast neutrons. Data on the frequency of chromosome aberrations and reduction in DNA content observed in root-tip nuclei indicated a possible interaction between polyploidy and the LET of the radiation employed. Seed fertility was less affected in polyploids than in diploids. The frequency of chlorophyll mutations decreased with increasing ploidy in both wheat and barley while the frequency of viable mutations registered the opposite trend in wheat. Recessive mutations could be detected in 4x barley only in the M/sub 3/ generation. The relative proportion of chlorophyll and morphological mutations observed in 4x barley during the M/sub 3/ generation was similar to that found in 6x wheat in M/sub 2/ and M/sub 3/ generations. Though a high frequency of viable mutations was observed in 6x wheat, the spectrum of the induced variation was very narrow, thereby indicating that this species is a functional diploid only for a few loci. (auth)},
doi = {},
url = {https://www.osti.gov/biblio/4800392}, journal = {Radiation Botany},
number = ,
volume = Vol: 1,
place = {Country unknown/Code not available},
year = {Mon Jan 01 00:00:00 EST 1962},
month = {Mon Jan 01 00:00:00 EST 1962}
}