Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line
- Univ. of Calgary, Alberta (Canada)
- Cross Cancer Institute, Edmonton, Alberta (Canada)
The radiosensitive rodent mutant cell lines xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair. 36 refs., 4 figs., 1 tab.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 47991
- Journal Information:
- Science, Vol. 267, Issue 5201; Other Information: PBD: 24 Feb 1995
- Country of Publication:
- United States
- Language:
- English
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