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Title: Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns

Abstract

The band dispersion phenomena in capillary zone electrophoresis (CZE) using untreated and surface-treated open tubular and gel-filled capillaries were experimentally evaluated, with emphasis on small capillary diameters (10-100 {mu}m). Laser-induced fluorescence detection was used for high-sensitivity detection of the isoindoles originated from model amino acids. The plots of plate height vs electric field strength were generated for different column radii and compared with a theoretical model for CZE. In addition to the diffusion-controlled band dispersion in the relatively low electric field range, adsorptive interactions between a solute and the capillary wall may play a certain role in band-broadening. The sorption-desorption kinetics become important with increasing electric field strength. Thermal effects appear to contribute little to band-broadening in relatively small capillaries (<50-{mu}m i.d.) within normal operating voltages (<30 kV), but could become significant in capillaries with larger bores (>75-{mu}m i.d.). With gel-filled capillaries of small diameters (<50-{mu}m i.d.), diffusion processes can be minimized. In addition, thermal effects do not appear critical in such columns at reasonable voltages. 29 refs., 12 figs., 1 tab.

Authors:
; ; ;  [1]
  1. Indiana Univ., Bloomington, IN (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
478786
DOE Contract Number:  
FG02-84ER60215
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington)
Additional Journal Information:
Journal Volume: 64; Journal Issue: 13; Other Information: PBD: 1 Jul 1992
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; INDOLES; ELECTROPHORESIS; FLUORESCENCE SPECTROSCOPY; CHEMICAL REACTION KINETICS; CAPILLARIES; LASER SPECTROSCOPY; MATHEMATICAL MODELS

Citation Formats

Liu, J, Dolnik, V, Hsieh, You-Zung, and Novotny, M. Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns. United States: N. p., 1992. Web. doi:10.1021/ac00037a006.
Liu, J, Dolnik, V, Hsieh, You-Zung, & Novotny, M. Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns. United States. https://doi.org/10.1021/ac00037a006
Liu, J, Dolnik, V, Hsieh, You-Zung, and Novotny, M. Wed . "Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns". United States. https://doi.org/10.1021/ac00037a006.
@article{osti_478786,
title = {Experimental evaluation of the separation efficiency in capillary electrophoresis using open tubular and gel-filled columns},
author = {Liu, J and Dolnik, V and Hsieh, You-Zung and Novotny, M},
abstractNote = {The band dispersion phenomena in capillary zone electrophoresis (CZE) using untreated and surface-treated open tubular and gel-filled capillaries were experimentally evaluated, with emphasis on small capillary diameters (10-100 {mu}m). Laser-induced fluorescence detection was used for high-sensitivity detection of the isoindoles originated from model amino acids. The plots of plate height vs electric field strength were generated for different column radii and compared with a theoretical model for CZE. In addition to the diffusion-controlled band dispersion in the relatively low electric field range, adsorptive interactions between a solute and the capillary wall may play a certain role in band-broadening. The sorption-desorption kinetics become important with increasing electric field strength. Thermal effects appear to contribute little to band-broadening in relatively small capillaries (<50-{mu}m i.d.) within normal operating voltages (<30 kV), but could become significant in capillaries with larger bores (>75-{mu}m i.d.). With gel-filled capillaries of small diameters (<50-{mu}m i.d.), diffusion processes can be minimized. In addition, thermal effects do not appear critical in such columns at reasonable voltages. 29 refs., 12 figs., 1 tab.},
doi = {10.1021/ac00037a006},
url = {https://www.osti.gov/biblio/478786}, journal = {Analytical Chemistry (Washington)},
number = 13,
volume = 64,
place = {United States},
year = {1992},
month = {7}
}