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Title: Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis

Abstract

The quantitative precision in capillary zone electrophoresis (CZE) with electrokinetic injection can be improved by proper correction with the integrated current during injection. By monitoring the electrophoretic current during separation, one can also correct for temporal fluctuations in the analyte migration velocity. A model based on field-amplified capillary electrophoresis is used to describe the electrokinetic injection process involving sample solutions with distinct conductivities, and an equation is developed to allow normalization of peak areas at the 0-5% level without the need of quantitative internal standardization. 28 refs., 2 figs., 2 tabs.

Authors:
;  [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
478546
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington)
Additional Journal Information:
Journal Volume: 64; Journal Issue: 11; Other Information: PBD: 1 Jun 1992
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; ELECTROPHORESIS; MATERIALS; SAMPLING; STANDARDIZATION; MATHEMATICAL MODELS

Citation Formats

Lee, T T, and Yeung, E S. Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis. United States: N. p., 1992. Web. doi:10.1021/ac00035a007.
Lee, T T, & Yeung, E S. Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis. United States. https://doi.org/10.1021/ac00035a007
Lee, T T, and Yeung, E S. Mon . "Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis". United States. https://doi.org/10.1021/ac00035a007.
@article{osti_478546,
title = {Compensating for instrumental and sampling biases accompanying electrokinetic injection in capillary zone electrophoresis},
author = {Lee, T T and Yeung, E S},
abstractNote = {The quantitative precision in capillary zone electrophoresis (CZE) with electrokinetic injection can be improved by proper correction with the integrated current during injection. By monitoring the electrophoretic current during separation, one can also correct for temporal fluctuations in the analyte migration velocity. A model based on field-amplified capillary electrophoresis is used to describe the electrokinetic injection process involving sample solutions with distinct conductivities, and an equation is developed to allow normalization of peak areas at the 0-5% level without the need of quantitative internal standardization. 28 refs., 2 figs., 2 tabs.},
doi = {10.1021/ac00035a007},
url = {https://www.osti.gov/biblio/478546}, journal = {Analytical Chemistry (Washington)},
number = 11,
volume = 64,
place = {United States},
year = {1992},
month = {6}
}