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Title: Microbial response to triepthylphosphate

Abstract

The effect of triethylphosphate (TEP) on the activity of a landfill aquifer microbial community was evaluated using standard techniques and in situ hybridizations with phylogenetic probes. Benzene was used as an external carbon source to monitor degradation of an aromatic compound in TEP amended microcosms. Microscopical and viable counts were higher in TEP containing microcosms when compared to unamended controls. A significant increase in metabolic activity was also observed for TEP amended samples as determined by the number of cells hybridizing to an eubacterial probe. In addition, the number of beta and gamma Proteobacteria increased from undetectable levels prior to the study to 15-29% of the total bacteria in microcosms containing TEP and benzene. In these microcosms, nearly 40% of the benzene was degraded during the incubation period compared to less than 5% in unamended microcosms. While TEP has previously been used as an alternate phosphate source in the bioremediation of chlorinated aliphatics, this study shows that it can also stimulate the microbial degradation of aromatics in phosphate limited aquifers.

Authors:
; ;
Publication Date:
Research Org.:
Westinghouse Savannah River Co., Aiken, SC (United States)
Sponsoring Org.:
USDOE, Washington, DC (United States)
OSTI Identifier:
477743
Report Number(s):
WSRC-MS-97-0003
ON: DE97060077; TRN: 97:003588
DOE Contract Number:
AC09-96SR18500
Resource Type:
Technical Report
Resource Relation:
Other Information: PBD: [1997]
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; AQUIFERS; REMEDIAL ACTION; BENZENE; BIODEGRADATION; METABOLISM; AROMATICS; BACTERIA; MICROCOSMS; PROBES; PHOSPHATES; ADDITIVES

Citation Formats

Hazen, T.C., Santo Domingo, J.W., and Berry, C.J.. Microbial response to triepthylphosphate. United States: N. p., 1997. Web. doi:10.2172/477743.
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Hazen, T.C., Santo Domingo, J.W., & Berry, C.J.. Microbial response to triepthylphosphate. United States. doi:10.2172/477743.
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Hazen, T.C., Santo Domingo, J.W., and Berry, C.J.. Thu . "Microbial response to triepthylphosphate". United States. doi:10.2172/477743. https://www.osti.gov/servlets/purl/477743.
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@article{osti_477743,
title = {Microbial response to triepthylphosphate},
author = {Hazen, T.C. and Santo Domingo, J.W. and Berry, C.J.},
abstractNote = {The effect of triethylphosphate (TEP) on the activity of a landfill aquifer microbial community was evaluated using standard techniques and in situ hybridizations with phylogenetic probes. Benzene was used as an external carbon source to monitor degradation of an aromatic compound in TEP amended microcosms. Microscopical and viable counts were higher in TEP containing microcosms when compared to unamended controls. A significant increase in metabolic activity was also observed for TEP amended samples as determined by the number of cells hybridizing to an eubacterial probe. In addition, the number of beta and gamma Proteobacteria increased from undetectable levels prior to the study to 15-29% of the total bacteria in microcosms containing TEP and benzene. In these microcosms, nearly 40% of the benzene was degraded during the incubation period compared to less than 5% in unamended microcosms. While TEP has previously been used as an alternate phosphate source in the bioremediation of chlorinated aliphatics, this study shows that it can also stimulate the microbial degradation of aromatics in phosphate limited aquifers.},
doi = {10.2172/477743},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu May 01 00:00:00 EDT 1997},
month = {Thu May 01 00:00:00 EDT 1997}
}
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Technical Report:
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DOI:  10.2172/477743
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