TWO-STEP TRANSPLANTATION THERAPY IN LETHALLY IRRADIATED MICE
Attempts were made to form mouse radiation chimeras potentially capable of undergoing further transplantation therapy and accepting grafts from a second donor, unrelated to both host and the first donor of the restorative cell graft of myeloid and lymphoid tissues. Different approaches were tested in an attempt to modify the immune reactivity conferred on the irradiated recipient with the restorative graft from the first donor by inducing tolerance to a prospective second donor. Male radiation sterility was found to be an unsatisfactory experimental model for transplantation therapy. It was also difficult to decide whether an intratesticular cell graft by itself would be able to induce tolerance or rather to escape immunity on the part of the chimera host. In further experiments, test grafts were either heterotopically transplanted ovaries in female radiation chimeras, skin grafts, or both. The behavior of ovarian tissue did not differ from that of skin when used as test-graft in presumably tolerant recipients. The tissues either both took or were both rejected. Survival of grafts from a second donor was obtained only when tolerance to its specific antigens was first induced in the restorative cell grait by an admixture of cells of its genotype or of pooled cells from many donors belonging to its antigenically heterogeneous mouse population. Solid tissue (skin) was not a satisfactory source of antigenic stimulus for the induction of graft-to-graft tolerance. The limits to the use of the principle of antigenic overlapping for the induction of polyvalent immunological tolerance of tissues from donors selected at random are discussed. (auth)
- Research Organization:
- Inst. of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague
- NSA Number:
- NSA-17-004432
- OSTI ID:
- 4758513
- Journal Information:
- Folia Biologica (Prague) (Czechoslovakia), Vol. Vol: 8; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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