THE EFFECT OF IRRADIATION ON NUCLEOTIDE METABOLISM IN RABBIT LIVER (in Russian)
A group of rabbits was given a whole-body dose of 1000 r from a Co/sup 60/ source. Tests were carried out on the rabbits 30 minutes, 4, 12, 24, and 48 hours after irradiation. The control rabbits and irradiated rabbits were given an intravenous dose of 0.1 mu C/g body weight of Na/sub 2/HP/sup 32/O/sub 4/, and were killed 15 minutes after being given the tracer. The nucleotide fraction was rapidly isolated from 50 g of liver tissue in the form of a barium salt. The nucleotides were separated by anion exchange on a Dowex 2 column in the formate form. The successive eluants used were H/sub 2/O, 4N HCOOH and a mixture of 4N HCOOH and 0.8N HCOONH/sub 4/. Paper chromatography was used to resolve the peaks further. The concentration of separate mononucleotides was calculated from absorption measurements. The results are given in micromoles per gram of dry liver. The P/sup 32/ content is expressed in counts per minute per micromole of nucleotide as related to the counts per minute per micromole of inorganic phosphate in the acid-soluble fraction of the tissue. The following substances were identified: adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine5-phosphoric acid (AMP-5), ionosine-5-phosphoric acid (IMP-5), guanosine triphosphate (GTP), guanosine diphosphate (GDP), guanosine-5-phosphoric acid (GMP- 5), uridine diphosphate (UDP), uridine-5-phosphoric acid (UMP-5), uridine diphosphate glucose (UDPG), uridine diphosphate acetyl glucosoamine (UDPAGA), uridine phosphate glucuronic acid (UPGA), cytidine-5-phosphoric acid (CMP-5), diphosphopyridine nucleotide (DPN), and triphosphopyridine nucleotide (TPN). Changes in the content of separate nucleotides were noted 30 minutes after irradiation. Thus, the content of IMP-5 and of CMP-5 increased by a factor of two as compared to the unirradiated controls. The concentration of monophosphate nucleosides was higher 24 hours after irradiation: GMP-5 by 40%, CMP-5 by 38% and UMP5 by 28% higher than normal. On the other hand, the concentration of triphosphate nucleosides was lower: ATP by 65% and GTP by 25% lower than normal. The nucleotide concentrations returned to normal levels two days after the irradiation. The rate of P/sup 32/ incorporation by diphosphate and triphosphate nucleosides increased by 30 to 50 percent 24 hours after irradiation. (TTT).
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-17-005879
- OSTI ID:
- 4753910
- Journal Information:
- Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Vol. Vol: 2; Other Information: Orig. Receipt Date: 31-DEC-63
- Country of Publication:
- Country unknown/Code not available
- Language:
- Russian
Similar Records
(5'-/sup 32/P)-8-azidoguanosine-3',5'-monophosphate. I. Synthesis and properties. II. Interaction with E. coli proteins
Suppression of the biosynthesis of guanosine triphosphate by protein synthesis inhibitors