Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax

doi:https://doi.org/10.1002/etc.5620160217

Title: Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax

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Abstract

The effect of copper chloride was studied on the hepatic microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of the fish Dicentrarchus labrax intraperitoneally injected with benzo[a]pyrene (BaP). In vitro experiments showed that copper significantly decreased EROD activity, and IC50 was estimated at 50 {micro}g Cu/L. The apparent Michaelis constant (K{sub m}) of cytochrome P4501A was constant, whereas maximum velocity (V{sub max}) decreased as a function of copper added to the incubation medium. (K{sub m}) of nicotinamide adenine dinucleotide phosphate [NADPH]-cytochrome P450 reductase increased as a function of copper concentration, whereas V{sub max} remained constant. Absorption spectra showed that the amount of cytochrome P420s increased as a function of copper concentrations added to the medium at the expense of cytochrome P450s. The injection of copper and BaP to fish decreased EROD activity compared with the injection of BaP alone. An increase of immunoquantified CYP1A content measured by Western blotting was noted in microsomes of fish injected with BaP compared with controls. In the case of fish treated with copper and BaP, the band was less intense and accompanied by another band of lower molecular weight. The destruction of the native P450s spectrophotometrically measured in the presence of copper implied that the catalytic activity wouldmore »« less

Authors:

Stien, X; Risso, C; Gnassia-Barelli, M; Romeo, M; LaFaurie, M ^[1]

Univ. de Nice-Sophia Antipolis, Nice (France)

Publication Date:: 1997-02-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 474321

Resource Type:: Journal Article

Journal Name:: Environmental Toxicology and Chemistry

Additional Journal Information:: Journal Volume: 16; Journal Issue: 2; Other Information: PBD: Feb 1997

Country of Publication:: United States

Language:: English

Subject:: 54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; BIOLOGICAL INDICATORS; COPPER; BENZOPYRENE; BIOLOGICAL MARKERS; TOXICITY; METABOLISM; ENZYME ACTIVITY; LIVER; OXIDOREDUCTASES; FISHES

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                    Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, and LaFaurie, M. Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax.  United States: N. p., 1997. 
        Web.  doi:10.1002/etc.5620160217.

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                    Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, & LaFaurie, M. Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax.  United States.  https://doi.org/10.1002/etc.5620160217

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                    Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, and LaFaurie, M. Sat .  
        "Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax".  United States.  https://doi.org/10.1002/etc.5620160217.

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@article{osti_474321,

  title        = {Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax},

  author       = {Stien, X and Risso, C and Gnassia-Barelli, M and Romeo, M and LaFaurie, M},

  abstractNote = {The effect of copper chloride was studied on the hepatic microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of the fish Dicentrarchus labrax intraperitoneally injected with benzo[a]pyrene (BaP). In vitro experiments showed that copper significantly decreased EROD activity, and IC50 was estimated at 50 {micro}g Cu/L. The apparent Michaelis constant (K{sub m}) of cytochrome P4501A was constant, whereas maximum velocity (V{sub max}) decreased as a function of copper added to the incubation medium. (K{sub m}) of nicotinamide adenine dinucleotide phosphate [NADPH]-cytochrome P450 reductase increased as a function of copper concentration, whereas V{sub max} remained constant. Absorption spectra showed that the amount of cytochrome P420s increased as a function of copper concentrations added to the medium at the expense of cytochrome P450s. The injection of copper and BaP to fish decreased EROD activity compared with the injection of BaP alone. An increase of immunoquantified CYP1A content measured by Western blotting was noted in microsomes of fish injected with BaP compared with controls. In the case of fish treated with copper and BaP, the band was less intense and accompanied by another band of lower molecular weight. The destruction of the native P450s spectrophotometrically measured in the presence of copper implied that the catalytic activity would be diminished. This was confirmed by decreased EROD activity after either in vitro additions or in vivo treatment with copper. Moreover, immunodetection experiments suggested that the decrease of the catalytic activity resulted more from cytochrome P450s loss than from direct inhibition of EROD activity by copper.},

  doi          = {10.1002/etc.5620160217},

  url          = {https://www.osti.gov/biblio/474321},
  journal      = {Environmental Toxicology and Chemistry},
number       = 2,

  volume       = 16,

  place        = {United States},

  year         = {1997},

  month        = {2}

}

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