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Title: Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax

Abstract

The effect of copper chloride was studied on the hepatic microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of the fish Dicentrarchus labrax intraperitoneally injected with benzo[a]pyrene (BaP). In vitro experiments showed that copper significantly decreased EROD activity, and IC50 was estimated at 50 {micro}g Cu/L. The apparent Michaelis constant (K{sub m}) of cytochrome P4501A was constant, whereas maximum velocity (V{sub max}) decreased as a function of copper added to the incubation medium. (K{sub m}) of nicotinamide adenine dinucleotide phosphate [NADPH]-cytochrome P450 reductase increased as a function of copper concentration, whereas V{sub max} remained constant. Absorption spectra showed that the amount of cytochrome P420s increased as a function of copper concentrations added to the medium at the expense of cytochrome P450s. The injection of copper and BaP to fish decreased EROD activity compared with the injection of BaP alone. An increase of immunoquantified CYP1A content measured by Western blotting was noted in microsomes of fish injected with BaP compared with controls. In the case of fish treated with copper and BaP, the band was less intense and accompanied by another band of lower molecular weight. The destruction of the native P450s spectrophotometrically measured in the presence of copper implied that the catalytic activity wouldmore » be diminished. This was confirmed by decreased EROD activity after either in vitro additions or in vivo treatment with copper. Moreover, immunodetection experiments suggested that the decrease of the catalytic activity resulted more from cytochrome P450s loss than from direct inhibition of EROD activity by copper.« less

Authors:
; ; ; ;  [1]
  1. Univ. de Nice-Sophia Antipolis, Nice (France)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
474321
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry
Additional Journal Information:
Journal Volume: 16; Journal Issue: 2; Other Information: PBD: Feb 1997
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; WATER POLLUTION; BIOLOGICAL INDICATORS; COPPER; BENZOPYRENE; BIOLOGICAL MARKERS; TOXICITY; METABOLISM; ENZYME ACTIVITY; LIVER; OXIDOREDUCTASES; FISHES

Citation Formats

Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, and LaFaurie, M. Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax. United States: N. p., 1997. Web. doi:10.1002/etc.5620160217.
Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, & LaFaurie, M. Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax. United States. https://doi.org/10.1002/etc.5620160217
Stien, X, Risso, C, Gnassia-Barelli, M, Romeo, M, and LaFaurie, M. Sat . "Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax". United States. https://doi.org/10.1002/etc.5620160217.
@article{osti_474321,
title = {Effect of copper chloride in vitro and in vivo on the hepatic EROD activity in the fish Dicentrarchus labrax},
author = {Stien, X and Risso, C and Gnassia-Barelli, M and Romeo, M and LaFaurie, M},
abstractNote = {The effect of copper chloride was studied on the hepatic microsomal 7-ethoxyresorufin-O-deethylase (EROD) activity of the fish Dicentrarchus labrax intraperitoneally injected with benzo[a]pyrene (BaP). In vitro experiments showed that copper significantly decreased EROD activity, and IC50 was estimated at 50 {micro}g Cu/L. The apparent Michaelis constant (K{sub m}) of cytochrome P4501A was constant, whereas maximum velocity (V{sub max}) decreased as a function of copper added to the incubation medium. (K{sub m}) of nicotinamide adenine dinucleotide phosphate [NADPH]-cytochrome P450 reductase increased as a function of copper concentration, whereas V{sub max} remained constant. Absorption spectra showed that the amount of cytochrome P420s increased as a function of copper concentrations added to the medium at the expense of cytochrome P450s. The injection of copper and BaP to fish decreased EROD activity compared with the injection of BaP alone. An increase of immunoquantified CYP1A content measured by Western blotting was noted in microsomes of fish injected with BaP compared with controls. In the case of fish treated with copper and BaP, the band was less intense and accompanied by another band of lower molecular weight. The destruction of the native P450s spectrophotometrically measured in the presence of copper implied that the catalytic activity would be diminished. This was confirmed by decreased EROD activity after either in vitro additions or in vivo treatment with copper. Moreover, immunodetection experiments suggested that the decrease of the catalytic activity resulted more from cytochrome P450s loss than from direct inhibition of EROD activity by copper.},
doi = {10.1002/etc.5620160217},
url = {https://www.osti.gov/biblio/474321}, journal = {Environmental Toxicology and Chemistry},
number = 2,
volume = 16,
place = {United States},
year = {1997},
month = {2}
}