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Title: EFFECT OF IONIZING RADIATION ON THE PYRIDINE NUCLEOTIDES OF THYMOCYTES

Abstract

The pyridine nucleotides of thymocytes were examined following irradiation of the cells in vivo or in vitro. Nicotinamide-adenine dinucleotide (NAD) was found to constitute about 95% of the total oxidized pyridine nucleotides in thymocytes while the level of NADH₂ was found to be very low. There is a rapid loss of NAD from thymocytes following irradiation in vitro or in vivo which is evident with as little as 25 rads. There is no concomitant rise in the NADH₂ levels following irradiation. The ioss of NAD occurs principally from the nucleus of the cell and is characterized by an intracellular release of NADase from the mitochondria and nucleus into the cytoplasm. Following irradiation there is a reduced capacity for thymocytes to synthesize NAD from 7-C¹⁴-nicotinamide either in vitro or in vivo. The administration or presence of nicotinamide produces an unphysiologicai increase in the level of NAD in whole thymus or thymocytes in suspension. The level of NAD in treated cells is higher than in untreated controls following irradiation. This effect is not produced by nicotinic acid, cysteamine, L-cysteine, or promethazine. The administration of nicotinamide does not prevent the development of pyknotic degeneration in irradiated thymocytes. There is no change in themore » RNA content of the thymus under the conditions which reduce the NAD content. (auth)« less

Authors:
Publication Date:
Research Org.:
Defence Research Chemical Labs., Ottawa
Sponsoring Org.:
USDOE
OSTI Identifier:
4716437
Report Number(s):
DRCL-402
Journal ID: ISSN 0829-8211
NSA Number:
NSA-17-027085
Resource Type:
Journal Article
Journal Name:
Biochemistry and Cell Biology
Additional Journal Information:
Journal Volume: 41; Journal Issue: 6; Other Information: DRCL-402. Orig. Receipt Date: 31-DEC-63; Journal ID: ISSN 0829-8211
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
BIOLOGY AND MEDICINE; ALKALOIDS; AMIDES; AMINES; ANIMAL CELLS; BIOCHEMISTRY; CARBON 14; CYSTEAMINE; CYSTEINE; CYTOLOGY; ENZYMES; HETEROCYCLICS; METHYL RADICALS; MITOCHONDRIA; NICOTINE; NUCLEIC ACIDS; ORGANIC NITROGEN COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PHENOTHIAZINES; PROPYL RADICALS; QUANTITY RATIO; RADIATION DOSES; RADIATION EFFECTS; RIBONUCLEIC ACID; THYMUS; TRACER TECHNIQUES

Citation Formats

Scaife, J. F. EFFECT OF IONIZING RADIATION ON THE PYRIDINE NUCLEOTIDES OF THYMOCYTES. Country unknown/Code not available: N. p., 1963. Web. doi:10.1139/o63-166.
Scaife, J. F. EFFECT OF IONIZING RADIATION ON THE PYRIDINE NUCLEOTIDES OF THYMOCYTES. Country unknown/Code not available. https://doi.org/10.1139/o63-166
Scaife, J. F. Tue . "EFFECT OF IONIZING RADIATION ON THE PYRIDINE NUCLEOTIDES OF THYMOCYTES". Country unknown/Code not available. https://doi.org/10.1139/o63-166.
@article{osti_4716437,
title = {EFFECT OF IONIZING RADIATION ON THE PYRIDINE NUCLEOTIDES OF THYMOCYTES},
author = {Scaife, J. F.},
abstractNote = {The pyridine nucleotides of thymocytes were examined following irradiation of the cells in vivo or in vitro. Nicotinamide-adenine dinucleotide (NAD) was found to constitute about 95% of the total oxidized pyridine nucleotides in thymocytes while the level of NADH₂ was found to be very low. There is a rapid loss of NAD from thymocytes following irradiation in vitro or in vivo which is evident with as little as 25 rads. There is no concomitant rise in the NADH₂ levels following irradiation. The ioss of NAD occurs principally from the nucleus of the cell and is characterized by an intracellular release of NADase from the mitochondria and nucleus into the cytoplasm. Following irradiation there is a reduced capacity for thymocytes to synthesize NAD from 7-C¹⁴-nicotinamide either in vitro or in vivo. The administration or presence of nicotinamide produces an unphysiologicai increase in the level of NAD in whole thymus or thymocytes in suspension. The level of NAD in treated cells is higher than in untreated controls following irradiation. This effect is not produced by nicotinic acid, cysteamine, L-cysteine, or promethazine. The administration of nicotinamide does not prevent the development of pyknotic degeneration in irradiated thymocytes. There is no change in the RNA content of the thymus under the conditions which reduce the NAD content. (auth)},
doi = {10.1139/o63-166},
url = {https://www.osti.gov/biblio/4716437}, journal = {Biochemistry and Cell Biology},
issn = {0829-8211},
number = 6,
volume = 41,
place = {Country unknown/Code not available},
year = {1963},
month = {1}
}