The X-Ray Sensitivity of Serratia marcescens
Serratia marcescens was irradiated with 200-Kv and 250-Kv x rays under a number of conditions of temperature, dose rate, and pH. The log surviving fraction was plotted against dose so that the slope and hit number of the inactivation curve could be measured in every case. The effect of bubbling the bacterial suspension with the following gases was noted: oxygen, nitrogen, nitric oxide, nitrous oxide, and hydrogen. The effect of the following substances at various concentrations in solution was also noted: glycerol, ethanol, ethylene glycol, cysteine, dimethyl sulfoxide, and N-ethylmaleimide. Various combinations of physical conditions, gases, and chemicals were investigated. In a plot of the log surviving fraction against dose, the survival curve in oxygen was found to be a straight line over many powers of 10. In nitrogen the survival curve was not straight. Three types of damage were deduced: damage observed by small doses in nitrogen, additional damage in oxygen, and a temperature-dependent postirradiation damage observed at high doses in nitrogen. Oxygen protects against postirradiation damage. Glycerol and other alcohols protect damage in both oxygen and nitrogen. N-Ethylmaleimide sensitizes the postirradiation type of damage. Cysteine protects against damage in nitrogen and the postirradiation damage but not against damage in oxygen. (auth)
- Research Organization:
- Mount Vernon Hospital, Northwood, Middx, Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-024968
- OSTI ID:
- 4714421
- Journal Information:
- Radiation Research, Vol. 19, Issue 1; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0033-7587
- Publisher:
- Radiation Research Society
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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