Parallel human genome analysis: Microarray-based expression monitoring of 1000 genes
- Stanford Univ. Medical Center, CA (United States); and others
Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm{sup 2} DNA {open_quotes}chips{close_quotes} were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery. 33 refs., 3 figs., 2 tabs.
- OSTI ID:
- 468347
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20; Other Information: PBD: 1 Oct 1996
- Country of Publication:
- United States
- Language:
- English
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