Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase
- Lund Univ. (Sweden); and others
By catalyzing the rate-limiting step in adipose tissue lipolysis, hormone-sensitive lipase (HSL) is an important regulator of energy homeostasis. The role and importance of HSL in tissues other than adipose are poorly understood. We report here the cloning and expression of a testicular isoform, designated HSL{sub tes}. Due to an addition of amino acids at the NH{sub 2}-termini, rat and human HSL{sub tes} consist of 1068 and 1076 amino acids, respectively, compared to the 768 and 775 amino acids, respectively, of the adipocyte isoform (HSL{sub adi}). A novel exon of 1.2 kb, encoding the human testis-specific amino acids, was isolated and mapped to the HSL gene, 16 kb upstream of the exons encoding HSL{sub adi}. The transcribed mRNA of 3.9 kb was specifically expressed in testis. No significant similarity with other known proteins was found for the testis-specific sequence. The amino acid composition differs from the HSL{sub adi} sequence, with a notable hydrophilic character and a high content of prolines and glutamines. COS cells, transfected by the 3.9-kb human testis cDNA, expressed a protein of the expected molecular mass (M{sub r} {approximately}120,000) that exhibited catalytic activity similar to that of HSL{sub adi}. Immunocytochemistry localized HSL to elongating spermatids and spermatozoa; HSL was not detected in interstitial cells. 34 refs., 5 figs.
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 465978
- Journal Information:
- Genomics, Vol. 35, Issue 3; Other Information: PBD: 1 Aug 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
LIPASES
DNA-CLONING
GENE REGULATION
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
TISSUE DISTRIBUTION
GENETIC MAPPING
TRANSCRIPTION
ENERGY
HOMEOSTASIS
HUMAN CHROMOSOME 19
HORMONES
ADIPOSE TISSUE
TESTES
AMINO ACIDS
PROLINE
GLUTAMINE
SPERMATOZOA
GENE AMPLIFICATION