Behavior of Noninfectious SV 40 Viral Genome in Hamster Tumor Cells: Induction of Synthesis of Infectious Virus
In transplanted SV 40 hamster tumors spontaneous development of infectious virus occurs only in an occasional cell out of many millions that carry the viral genome in a noninfectious state, more frequently in older tumors than in those that have grown for less than four weeks No infectious nucleic acid capable of producing complete SV 40 virus in susceptible Cercopithecus cells was found in the tumor cells. Induction of a greater frequency of synthesis of infectious virus in tumor cells has now been demonstrated by three procedures - propagation of the tumor cells in vitro --more frequently in rolled than in stationary cultures, although the latter are more suitable for optimum growth of the tumor cells; by association of viable tumor cells with susceptible cells under certain conditions of contact which do not require either proliferation or persistence of the tumor cells; by an x-ray effect on a very small proportion of the tumor cells. SV 40 tumors transplanted in newborn hamsters yield a satisfactory complement-fixing antigen which reacts specifically only with the sera of hamsters bearing SV 40 tumors but not with sera of hamsters bearing either polyoma tumors or Fortner fibrosarcoma no. 3 tumors. Hamsters bearing transplanted SV 40 tumors for four weeks or longer also develop complement-fixing antibody for an antigen present in BS-C -1 Cercopithecus cells infected with SV 40 virus, although these sera contain no neutralizing antibodies. Adult hamsters inoculated with large doses of SV 46 virus, which induces a specific resistence to transplantation of SV 40 tumor cells, possess no antibody for the antigen in the tumor or the antigen in the SV 40 virus-infected Cercopithecus cells. The available data are not sufffcient for a conclusion that the noninfectious viral genome in the SV 40 tumor cells carries information for the synthesis of at least a part of the viral antigen, although it gives rise to antigens that are specific for the SV 40 virus. The data on induction of infectious virus in the SV 40 tumor cells provide a new approach to the search for etiologically significant, dormant viruses in human cancers.
- Research Organization:
- Univ. of Cincinnati, OH (United States)
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-040464
- OSTI ID:
- 4624113
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 50, Issue 3; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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