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Title: Cytotoxic and genotoxic effects of some energetic compounds tested using various bacterial and mammalian cell assays

Abstract

Several energetic compounds (EC) present in explosive-contaminated soil and water samples, namely trinitrotoluene (TNT), cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and various amino and nitro derivatives of toluene were evaluated. The authors are studying cyto- and genotoxic effects of the EC using the Fluctuation Test (FT), the SOS Chromotest (SC) and the V79 mammalian cell mutagenicity assay (MC). They have used the neutral red cytotoxicity test with V79 cells as a screening tool before conducting the mutagenicity assays. In the V79 cell assay, only 3,4-dinitrotoluene was found more toxic than TNT. RDX was clearly less toxic than TNT and HMX was not toxic for V79 cells at its maximal solubility limit. TNT metabolites were generally less toxic than the parent compound. The preliminary results with the FT, SC and MC assays indicate that TNT and 2,4-DNT are genotoxic using the FT, an effect which was decreased following the addition of S9. On the other hand, none of the agents tested were mutagenic using the SC. Lengthening the exposure time in the MC allowed for detection of a weak genotoxic activity of TNT and RDX. The authors are continuing studies in order to verify if the mammalian cell assay can be used tomore » further characterize the potential risk for wildlife and man in contaminated sites.« less

Authors:
; ; ; ; ;  [1]; ;  [2]
  1. BRI, Montreal, Quebec (Canada)
  2. DREV, Courcelette, Quebec (Canada)
Publication Date:
OSTI Identifier:
458294
Report Number(s):
CONF-961149-
Journal ID: ISSN 1087-8939; TRN: IM9717%%80
Resource Type:
Conference
Resource Relation:
Conference: 17. annual meeting of the Society of Environmental Toxicology and Chemistry: partnerships for the environment - science, education, and policy, Washington, DC (United States), 17-21 Nov 1996; Other Information: PBD: 1995; Related Information: Is Part Of SETAC 17. annual meeting -- Abstract book. Partnerships for the environment: Science, education, and policy; PB: 378 p.
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 45 MILITARY TECHNOLOGY, WEAPONRY, AND NATIONAL DEFENSE; 56 BIOLOGY AND MEDICINE, APPLIED STUDIES; NITRO COMPOUNDS; LAND POLLUTION; MUTAGEN SCREENING; GENETIC EFFECTS; EXPLOSIVES

Citation Formats

Lachance, B, Hawari, J, Sharmeen, N, Dodard, S, Robidoux, P Y, Sunahara, G, Ampleman, G, and Thiboutot, S. Cytotoxic and genotoxic effects of some energetic compounds tested using various bacterial and mammalian cell assays. United States: N. p., 1995. Web.
Lachance, B, Hawari, J, Sharmeen, N, Dodard, S, Robidoux, P Y, Sunahara, G, Ampleman, G, & Thiboutot, S. Cytotoxic and genotoxic effects of some energetic compounds tested using various bacterial and mammalian cell assays. United States.
Lachance, B, Hawari, J, Sharmeen, N, Dodard, S, Robidoux, P Y, Sunahara, G, Ampleman, G, and Thiboutot, S. Sun . "Cytotoxic and genotoxic effects of some energetic compounds tested using various bacterial and mammalian cell assays". United States.
@article{osti_458294,
title = {Cytotoxic and genotoxic effects of some energetic compounds tested using various bacterial and mammalian cell assays},
author = {Lachance, B and Hawari, J and Sharmeen, N and Dodard, S and Robidoux, P Y and Sunahara, G and Ampleman, G and Thiboutot, S},
abstractNote = {Several energetic compounds (EC) present in explosive-contaminated soil and water samples, namely trinitrotoluene (TNT), cyclotrimethylenetrinitramine (RDX), cyclotetramethylenetetranitramine (HMX), and various amino and nitro derivatives of toluene were evaluated. The authors are studying cyto- and genotoxic effects of the EC using the Fluctuation Test (FT), the SOS Chromotest (SC) and the V79 mammalian cell mutagenicity assay (MC). They have used the neutral red cytotoxicity test with V79 cells as a screening tool before conducting the mutagenicity assays. In the V79 cell assay, only 3,4-dinitrotoluene was found more toxic than TNT. RDX was clearly less toxic than TNT and HMX was not toxic for V79 cells at its maximal solubility limit. TNT metabolites were generally less toxic than the parent compound. The preliminary results with the FT, SC and MC assays indicate that TNT and 2,4-DNT are genotoxic using the FT, an effect which was decreased following the addition of S9. On the other hand, none of the agents tested were mutagenic using the SC. Lengthening the exposure time in the MC allowed for detection of a weak genotoxic activity of TNT and RDX. The authors are continuing studies in order to verify if the mammalian cell assay can be used to further characterize the potential risk for wildlife and man in contaminated sites.},
doi = {},
journal = {},
issn = {1087-8939},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
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