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Title: Analysis of petroleum-contaminated soils by thin-layer chromatography with flame ionization detection

Abstract

Thin-layer chromatography with flame ionization detection (TLC-FID) offers a rapid and accurate method for the analysis of non-volatile organic materials. If the instrument is properly calibrated and the operational parameters are standardized, TLC-FID permits a rapid and accurate quantification of total petroleum hydrocarbons (TPH), and offers the possibility of separation and quantification of the major types of components in petroleum and petroleum products. Petroleum hydrocarbons from contaminated soil were extracted overnight with a mixture of chloroform:methanol. The analysis method consisted of spotting a small volume of the samples on silica-coated quartz rods followed by three chromatographic developments in solvent systems of increasing polarities. This series of chromatographic steps resulted in the complete separation of aliphatic hydrocarbons, aromatic hydrocarbons, resins and asphaltenes, starting from mixtures of the materials. This method also allows the quantification of aromatic and aliphatic hydrocarbons without interference from biogenic lipids present in the soil. A simplified version of this method allowed excellent separation of aliphatics plus aromatics (forming a single peak) from resins and asphaltenes. The instrument provides the complete analysis of 10 samples in about one hour after extraction. This rapid analytical tool is especially suited for synoptic studies or for the screening of a largemore » number of samples prior to the consideration of more detailed and costly analyses.« less

Authors:
;  [1]
  1. Oak Ridge National Lab., TN (United States)
Publication Date:
OSTI Identifier:
452112
Report Number(s):
CONF-961149-
Journal ID: ISSN 1087-8939; TRN: IM9715%%144
Resource Type:
Conference
Resource Relation:
Conference: 17. annual meeting of the Society of Environmental Toxicology and Chemistry: partnerships for the environment - science, education, and policy, Washington, DC (United States), 17-21 Nov 1996; Other Information: PBD: 1995; Related Information: Is Part Of SETAC 17. annual meeting -- Abstract book. Partnerships for the environment: Science, education, and policy; PB: 378 p.
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; 54 ENVIRONMENTAL SCIENCES; THIN-LAYER CHROMATOGRAPHY; PETROLEUM; SOILS; OIL SPILLS; ENVIRONMENTAL IMPACTS; MEASURING INSTRUMENTS; TECHNOLOGY ASSESSMENT

Citation Formats

Napolitano, G E, and Richmond, J E. Analysis of petroleum-contaminated soils by thin-layer chromatography with flame ionization detection. United States: N. p., 1995. Web.
Napolitano, G E, & Richmond, J E. Analysis of petroleum-contaminated soils by thin-layer chromatography with flame ionization detection. United States.
Napolitano, G E, and Richmond, J E. Sun . "Analysis of petroleum-contaminated soils by thin-layer chromatography with flame ionization detection". United States.
@article{osti_452112,
title = {Analysis of petroleum-contaminated soils by thin-layer chromatography with flame ionization detection},
author = {Napolitano, G E and Richmond, J E},
abstractNote = {Thin-layer chromatography with flame ionization detection (TLC-FID) offers a rapid and accurate method for the analysis of non-volatile organic materials. If the instrument is properly calibrated and the operational parameters are standardized, TLC-FID permits a rapid and accurate quantification of total petroleum hydrocarbons (TPH), and offers the possibility of separation and quantification of the major types of components in petroleum and petroleum products. Petroleum hydrocarbons from contaminated soil were extracted overnight with a mixture of chloroform:methanol. The analysis method consisted of spotting a small volume of the samples on silica-coated quartz rods followed by three chromatographic developments in solvent systems of increasing polarities. This series of chromatographic steps resulted in the complete separation of aliphatic hydrocarbons, aromatic hydrocarbons, resins and asphaltenes, starting from mixtures of the materials. This method also allows the quantification of aromatic and aliphatic hydrocarbons without interference from biogenic lipids present in the soil. A simplified version of this method allowed excellent separation of aliphatics plus aromatics (forming a single peak) from resins and asphaltenes. The instrument provides the complete analysis of 10 samples in about one hour after extraction. This rapid analytical tool is especially suited for synoptic studies or for the screening of a large number of samples prior to the consideration of more detailed and costly analyses.},
doi = {},
journal = {},
issn = {1087-8939},
number = ,
volume = ,
place = {United States},
year = {1995},
month = {12}
}

Conference:
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