Crystal structure of DNA photolyase from Escherichia coli
- Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
- Univ. of North Carolina School of Medicine, Chapel Hill, NC (United States); and others
Photolyase repairs ultraviolet (UV) damage to DNA by splitting the cyclobutane ring of the major UV photoproduct, the cis,syn-cyclobutane pyrimidine dimer (Pyr<>Pyr). The reaction is initiated by blue light and proceeds through long-range energy transfer, single electron transfer, and enzyme catalysis by a radical mechanism. The three-dimensional crystallographic structure of DNA photolyase from Escherichia coli is presented and the atomic model was refined to an R value of 0.172 at 2.3 {angstrom} resolution. The polypeptide chain of 471 amino acids is folded into an amino-terminal {alpha}/{beta} domain resembling dinucleotide binding domains and a carboxyl-terminal helical domain; a loop of 72 residues connects the domains. The light-harvesting cofactor 5,10-methenyltetrahydrofolylpolyglutamate (MTHF) binds in a cleft between the two domains. Energy transfer from MTHF to the catalytic cofactor flavin adenine dinucleotide (FAD) occurs over a distance of 16.8 {angstrom}. The FAD adopts a U-shaped conformation between two helix clusters in the center of the helical domain and is accessible through a hole in the surface of this domain. Dimensions and polarity of the hole match those of a Pyr<>Pyr dinucleotide, suggesting that the Pyr<>Pyr {open_quotes}flips out{close_quotes} of the helix to fit into this hole, and that electron transfer between the flavin and the Pyr<>Pyr occurs over van der Waals contact distance. 56 refs., 5 figs., 2 tabs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 450605
- Journal Information:
- Science, Vol. 268, Issue 5219; Other Information: PBD: 30 Jun 1995
- Country of Publication:
- United States
- Language:
- English
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