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Title: Modulation of rabbit testicular contractions by prostaglandins, steroids, and some pharmacological compounds

Authors:
; ; ;
Publication Date:
Research Org.:
Originating Research Org. not identified
OSTI Identifier:
4489975
Report Number(s):
COO-1602-35
NSA Number:
NSA-28-005014
DOE Contract Number:
AT(11-1)-1602
Resource Type:
Technical Report
Resource Relation:
Other Information: Orig. Receipt Date: 31-DEC-73
Country of Publication:
United States
Language:
English
Subject:
N80700* -General & Miscellaneous; N48520 -Life Sciences- Radiation Effects on Animals-Vertebrates; *TESTES- PHYSIOLOGY; ADDITIVES; BIOLOGICAL EFFECTS; HORMONES; IN VITRO; RABBITS; STEROIDS

Citation Formats

Seeley, R.R., Hargrove, J.L., Johnson, J.M., and Ellis, L.C.. Modulation of rabbit testicular contractions by prostaglandins, steroids, and some pharmacological compounds. United States: N. p., 1972. Web. doi:10.2172/4489975.
Seeley, R.R., Hargrove, J.L., Johnson, J.M., & Ellis, L.C.. Modulation of rabbit testicular contractions by prostaglandins, steroids, and some pharmacological compounds. United States. doi:10.2172/4489975.
Seeley, R.R., Hargrove, J.L., Johnson, J.M., and Ellis, L.C.. Mon . "Modulation of rabbit testicular contractions by prostaglandins, steroids, and some pharmacological compounds". United States. doi:10.2172/4489975. https://www.osti.gov/servlets/purl/4489975.
@article{osti_4489975,
title = {Modulation of rabbit testicular contractions by prostaglandins, steroids, and some pharmacological compounds},
author = {Seeley, R.R. and Hargrove, J.L. and Johnson, J.M. and Ellis, L.C.},
abstractNote = {},
doi = {10.2172/4489975},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jun 26 00:00:00 EDT 1972},
month = {Mon Jun 26 00:00:00 EDT 1972}
}

Technical Report:

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  • The tonus and rate of spontaneous contractions measured in vivo from the tunica albuginea of rabbit testes were augmented by epinephrine, acetylcholine and prostaglandin (PG)F/sub 2 alpha/. Neither reserpine, dibenamine nor atropine decreased autorhythmic contractility. Indomethacin injections ip significantly decreased the mean amplitude of testicular contractions. This treatment effaced motility in two of eleven preparations. Injections of 5,8,11,14-eicosatetraynoic acid (TYA) did not significantly decrease the amplitude of contraction. Electrical stimulation of the spermatic artery (site of the spermatic nerve) in rabbits and in rats failed to alter testicular tonus or motility. The above evidence suggests that local factors such asmore » PGs may contribute more to smooth muscle activity in the tunica albuginea of rabbit testes than do autonomic nerves. The rhythmical contractions observed in vivo, however, continued in the presence of indomethacin in the bathing medium, and may not require stimulation by PGs for their origination. Rat testes, unlike rabbit testes, did not contract rhythmically in vivo. Isolated rat testes contracted in response to administered PGF/2 alpha/, while PGE/sub 1/ abolished the induced contraction. Such modulators of muscular tonus feasibly could alter intratesticular pressure for the intact animal.« less
  • Intoxication with nerve agents produces prolonged central nervous system seizures (status epilepticus) that can produce irreversible brain pathology (15). This report summarizes our recent findings regarding the neurotransmitter changes that occur in discrete brain regions as a function of seizure duration and the differential effectiveness of anticholinergic, benzodiazepine and excitatory amino acid (EAA) antagonist drugs in terminating soman-induced seizures when given at different times after seizure onset. These results are discussed in relation to a model we have proposed to explain the sequence of electrophysiological, biochemical and neurochemical events and mechanisms controlling nerve agent-induced seizures.
  • Highlights of progress achieved during the year ending February 28, 1983, are summarized. The long-range objective is to develop new rapid methods for the introduction of short-lived radionuclides into agents for use in diagnostic nuclear medicine. Methods have been developed to synthesize a radioiodine labeled fatty acid and a radioiodine labeled steroid. New methods have also been developed for introducing nitrogen isotopes into physiologically active materials and for the introduction of radiobromine into physiologically active materials.
  • N-methyl-D,L-aspartic acid (NMA), an agonist of the neurotransmitter glutamate has been shown to acutely stimulate the release of prolactin (PRL) in intact rats and monkeys. To further investigate the role of neuroexcitatory amino acids in PRL secretion, the effects of NMA administration were examined on PRL release in long term orchidectomized adult rhesus monkeys, in both the absence and presence of testosterone. Intact and long term castrated adult male monkeys weighing between 8-13 kg, were implanted with a catheter via the saphenous vein for blood withdrawal and drug infusion. Blood samples were collected at 10 min intervals for 50 minmore » before and 70 min after administration of the drug or vehicle. Plasma PRL concentrations were estimated using radioimmunoassay. Whereas a single iv injection of NMA induced a prompt discharge of PRL in intact monkeys, an identical dose had surprisingly no effect on PRL secretion in orchidectomized animals. On the other hand, plasma PRL increases in response to a challenge dose of thyrotropin releasing hormone were similar in magnitude in the two groups of monkeys. Testosterone replacement in orchidectomized animals by parenteral administration of testosterone enanthate reinitiated the PRL responsiveness to acute NMA stimulation. These results indicate that N-methyl-D-aspartic acid (NMDA) dependent drive to PRL release in the adult male rhesus monkey may be overtly influenced by the sex steroid milieu.« less