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Title: Occurrence and characteristics of {sup18}O-exchange reactions catalyzed by sodium- and potassium-dependent adenosine triphosphatases

Abstract

Microsomal preparations of the (NA{sup+},K{sup+})-ATPase from porcine outer medulla and electroplax catalyzed a rapid Mg{sup2+-} and K{sub+-}dependent exchange of water oxygens with inorganic phosphate in the absence of ATP or ADP. Exchange activity was unaffected by uncouplers and inhibitors of oxidative phosphorylation but was inhibited by Na{sup+}, ouabain, N,N{prime}-dicyclohexylcarbodiimide, or p-mercuribenzoate. No nucleotide requirement for the exchange could be demonstrated. Addition of ATP to a NA{sup+}-inhibited system resulted in an exchange of oxygens of medium P{subi} concomitant with ATP hydrolysis. This ATP-induced exchange amounted 1.5 oxygen atoms per P{subi} released. Neither ADP nor adenylyl methylene diphosphonate would serve to activate the exchange. Treatment of the preparations with deoxycholate apparently uncoupled the medium P{subi} {l_reversible} HOH exchange but was without affect on the ATP-induced exchange. The data suggest that the exchange arises by partial reversal of a late step in ATP hydrolysis by the (Na{sup+},K{sup+})-ATPase.

Authors:
;
Publication Date:
Research Org.:
University of California, Los Angeles (USA)
Sponsoring Org.:
US - Atomic Energy Commission (AEC)
OSTI Identifier:
4485348
Report Number(s):
UCLA-34-P-102-36
NSA Number:
NSA-28-005275
DOE Contract Number:  
AT(04-3)-34
Resource Type:
Technical Report
Resource Relation:
Other Information: Orig. Receipt Date: 31-DEC-73
Country of Publication:
United States
Language:
English
Subject:
N40230* -Chemistry-Inorganic, Organic, & Physical Chemistry-Isotope Exchange & Isotope Separation; *OXYGEN 18- ISOTOPIC EXCHANGE; ATP-ASE; CATALYSIS; CHEMICAL REACTION KINETICS; POTASSIUM COMPOUNDS; SODIUM COMPOUNDS; WATER

Citation Formats

Dahms, A.S., and Boyer, P.D. Occurrence and characteristics of {sup18}O-exchange reactions catalyzed by sodium- and potassium-dependent adenosine triphosphatases. United States: N. p., 1972. Web. doi:10.2172/4485348.
Dahms, A.S., & Boyer, P.D. Occurrence and characteristics of {sup18}O-exchange reactions catalyzed by sodium- and potassium-dependent adenosine triphosphatases. United States. doi:10.2172/4485348.
Dahms, A.S., and Boyer, P.D. Sat . "Occurrence and characteristics of {sup18}O-exchange reactions catalyzed by sodium- and potassium-dependent adenosine triphosphatases". United States. doi:10.2172/4485348. https://www.osti.gov/servlets/purl/4485348.
@article{osti_4485348,
title = {Occurrence and characteristics of {sup18}O-exchange reactions catalyzed by sodium- and potassium-dependent adenosine triphosphatases},
author = {Dahms, A.S. and Boyer, P.D.},
abstractNote = {Microsomal preparations of the (NA{sup+},K{sup+})-ATPase from porcine outer medulla and electroplax catalyzed a rapid Mg{sup2+-} and K{sub+-}dependent exchange of water oxygens with inorganic phosphate in the absence of ATP or ADP. Exchange activity was unaffected by uncouplers and inhibitors of oxidative phosphorylation but was inhibited by Na{sup+}, ouabain, N,N{prime}-dicyclohexylcarbodiimide, or p-mercuribenzoate. No nucleotide requirement for the exchange could be demonstrated. Addition of ATP to a NA{sup+}-inhibited system resulted in an exchange of oxygens of medium P{subi} concomitant with ATP hydrolysis. This ATP-induced exchange amounted 1.5 oxygen atoms per P{subi} released. Neither ADP nor adenylyl methylene diphosphonate would serve to activate the exchange. Treatment of the preparations with deoxycholate apparently uncoupled the medium P{subi} {l_reversible} HOH exchange but was without affect on the ATP-induced exchange. The data suggest that the exchange arises by partial reversal of a late step in ATP hydrolysis by the (Na{sup+},K{sup+})-ATPase.},
doi = {10.2172/4485348},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1972},
month = {Sat Jan 01 00:00:00 EST 1972}
}

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