Genetic mapping of a gene encoding an atypical protein kinase C, protein kinase C lambda, to the proximal region of mouse chromosome 3
- Imperial College of Science, Technology and Medicine, London (United Kingdom)
- CNR, Naples (Italy); and others
Protein kinase C are a class of proteins that mediate the control of cellular responses to external stimuli through the phosphorylation of target proteins. These proteins show a molecular heterogeneity by which it is possible to subdivide them into three subclasses (conventional, novel, and atypical) on the basis of their distinct structural and biochemical properties. We have recently described the isolation of a human gene encoding a new member of the atypical class of protein kinase C, protein kinase C{sub l} (PKC{sub l}), which we showed mapped to Xq21.3, close to the BTK gene. The atypical class of protein kinase C contains at least three members, protein kinase C{lambda}, protein kinase C{zeta}, and the previously mentioned protein kinase C{sub l}, although further members have been predicted to exist on the basis of genomic Southern blotting. To clone the mouse genes for new members of the atypical class of protein kinase C, we screened a mouse 11.5-day embryonic cDNA library with a 2.2-kb cDNA representing the entire human PKC{sub l} gene. Eight cDNA clones were isolated, of which six were either partially or fully sequenced. Their sizes ranged from 2.7 to 2.9 kb and appeared to be overlapping fragments of the same transcript. A FASTA search of the GenBank database using 1.5 kb of consensus cDNA sequence, containing mainly 3{prime} untranslated sequence, revealed 99% identity at the nucleotide level with mouse protein kinase C{lambda} (Accession No. D28577). Protein kinase C{lambda} (PKC{lambda}) has been previously characterized by Akimoto et al., but the gene encoding it had not been mapped in mouse or human. Mouse PKC{lambda} is expressed abundantly in undifferentiated P19 mouse embryonal carcinoma cells and shows a decrease in expression with differentiation. It is known to be expressed in a wide variety of tissues, but its precise biological function has yet to be elucidated. 8 refs., 1 fig.
- OSTI ID:
- 443894
- Journal Information:
- Genomics, Vol. 29, Issue 3; Other Information: PBD: 10 Oct 1995
- Country of Publication:
- United States
- Language:
- English
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