Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation
Abstract
The effect of single doses of/sup 137/Cs gamma rays or continuous /sup 60/Co gamma rays on mouse hemopoietic colony-forming units (CFU) was investigated using both the in vivo spleen colony assay technique (CFU/sub s/,) and the in vitro colony assay technique (CFU/sub c/). The difference in the respective D/sub o/ slopes was maintained during continuous irradiation. At the dose rate used (3.2 rad/hr) the shoulder on the CFU/sub s/ survival curve was abolished as expected, but the slopes of the early portions of the survival curves for both CFU/sub s/, and CFU/sub c/ were similar to those obtained after acute irradiation (600 rad/ min). A CFU/sub c/ subpopulation, which appears after adding red blood cells to the culture, showed a similar acute radiosensitivity to the whole CFU/sub c/ population but was less depleted under continuous irradiation. The results are discussed in relation to the kinetics of progenitor hemopoietic cell populations after a single, or during a prolonged, stress. (FR)
- Authors:
- Publication Date:
- Research Org.:
- Christie Hospital and Holt Radium Inst., Manchester, Eng.
- OSTI Identifier:
- 4424155
- NSA Number:
- NSA-29-000534
- Resource Type:
- Journal Article
- Journal Name:
- Biomedicine, v. 19, no. 5, pp. 183-186
- Additional Journal Information:
- Other Information: Orig. Receipt Date: 30-JUN-74
- Country of Publication:
- France
- Language:
- English
- Subject:
- N48210* -Life Sciences-Radiation Effects on Cells- External Source; *CFU- BIOLOGICAL RADIATION EFFECTS; *HEMATOPOIETIC SYSTEM- CFU; ACUTE IRRADIATION; BONE MARROW; CELL CULTURES; CELL PROLIFERATION; CHRONIC IRRADIATION; DOSE RATES; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; MICE; PHOTON BEAMS; RADIATION DOSES; SPLEEN COLONY FORMATION; STEM CELLS; SURVIVAL CURVES
Citation Formats
Testa, N G, Hendry, J H, and Lajtha, L G. Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation. France: N. p., 1973.
Web.
Testa, N G, Hendry, J H, & Lajtha, L G. Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation. France.
Testa, N G, Hendry, J H, and Lajtha, L G. 1973.
"Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation". France.
@article{osti_4424155,
title = {Response of mouse haemopoietic colony formers to acute or continuous gamma irradiation},
author = {Testa, N G and Hendry, J H and Lajtha, L G},
abstractNote = {The effect of single doses of/sup 137/Cs gamma rays or continuous /sup 60/Co gamma rays on mouse hemopoietic colony-forming units (CFU) was investigated using both the in vivo spleen colony assay technique (CFU/sub s/,) and the in vitro colony assay technique (CFU/sub c/). The difference in the respective D/sub o/ slopes was maintained during continuous irradiation. At the dose rate used (3.2 rad/hr) the shoulder on the CFU/sub s/ survival curve was abolished as expected, but the slopes of the early portions of the survival curves for both CFU/sub s/, and CFU/sub c/ were similar to those obtained after acute irradiation (600 rad/ min). A CFU/sub c/ subpopulation, which appears after adding red blood cells to the culture, showed a similar acute radiosensitivity to the whole CFU/sub c/ population but was less depleted under continuous irradiation. The results are discussed in relation to the kinetics of progenitor hemopoietic cell populations after a single, or during a prolonged, stress. (FR)},
doi = {},
url = {https://www.osti.gov/biblio/4424155},
journal = {Biomedicine, v. 19, no. 5, pp. 183-186},
number = ,
volume = ,
place = {France},
year = {Tue May 01 00:00:00 EDT 1973},
month = {Tue May 01 00:00:00 EDT 1973}
}