Lactic dehydrogenase in the tissues of cervical cancer under radiation therapy (in Japanese)
Variation in the isozyme pattern of lactic dehydrogenase (LDH) in the tissues of cervical cancer under radiation therapy was studied on 23 cases. The LDH isozyme patterns were classified into three groups. The LDH was divided into I to V fractions. The group A (13 cases 156.5%)) showed a decline in the order of LDH 1, 11, III, IV, and LDH V, the group B (seven cases 130-4%)) had its peak in LDH II and the group C (three cases 113-0%)) in LDH III. Marked variation in the isozyme pattern under radiation therapy occurred only in the group A. The LDH I showed a decline along with the radiation dosage; on the contrary LDH III and LDH IV increased. The M/H ratio, LDH I/LDH II ratio, and LDH I/LDH III ratio also showed a decline in parallel with the above variation, shifting over to the hybrid type from M type. In the group B and C, little variation was observed. (auth) Repair of uv-induced lesions in DNA was studied with various kinds of subcellular system prepared from Bacillus subtilis. The degree of repair during the post-irradiatlon incubation period was calculated from marker survivals in transforming DNA. Systems consisting mainly of non-viable spherical cells and subcellular fragments, as well as systems consisting of colony-forming protoplasts, were able to repair uv-induced lesions as efficiently as intact cell systems. A Teflon homogenate, a freeze-and-thawed product, and an osmotic shockate were also examined. The former two systems showed high repair activity, but the last did not. Attempts to repair the lesions with a supernatant fraction of Teflon homogenate were unsuccessful. In contrast with the active protoplast derivatives, toluene-treated cells were inert with respect to repair even when supplemented with substrates and cofactors although they retained DHA- synthesizing activity under similar conditions. (auth)
- Research Organization:
- Iwate Medical Coll., Morioka, Japan
- NSA Number:
- NSA-29-010348
- OSTI ID:
- 4393385
- Journal Information:
- Gan No Rinsho, v. 19, no. 3, pp. 223-230, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: JA (JA)
- Country of Publication:
- Japan
- Language:
- Japanese
Similar Records
In vitro study of cell survival following dynamic MLC intensity-modulated radiation therapy dose delivery
Photo-induced DNA damage, DNA repair and cell lethality