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Title: Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae

Abstract

A manganese peroxidase (mnp1) from Phanerochaete chrysosporium was efficiently expressed in Aspergillus oryzae. Expression was achieved by fusing the mature cDNA of mnp1 with the A. oryzae Taka amylase promoter and secretion signal. The 3{prime} untranslated region of the glucoamylase gene of Asperigillus awamori provided the terminator. The recombinant protein (rMnP) was secreted in an active form, permitting rapid detection and purification. Physical and kinetic properties of rMnP were similar to those of the native protein. The A. oryzae expression system is well suited for both mechanistic and site-directed mutagenesis studies. 34 refs., 7 figs., 1 tab.

Authors:
 [1]; ;  [2]
  1. Univ. of Wisconsin, Madison, WI (United States)
  2. Pennsylvanis State Univ., University Park, PA (United States) [and others
Publication Date:
OSTI Identifier:
433558
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; Journal Volume: 62; Journal Issue: 3; Other Information: PBD: Mar 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; ASPERGILLUS; GENE RECOMBINATION; PHANEROCHAETE; GENES

Citation Formats

Stewart, P., Whitwam, R.E., and Tien, Ming. Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. United States: N. p., 1996. Web.
Stewart, P., Whitwam, R.E., & Tien, Ming. Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. United States.
Stewart, P., Whitwam, R.E., and Tien, Ming. Fri . "Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae". United States.
@article{osti_433558,
title = {Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae},
author = {Stewart, P. and Whitwam, R.E. and Tien, Ming},
abstractNote = {A manganese peroxidase (mnp1) from Phanerochaete chrysosporium was efficiently expressed in Aspergillus oryzae. Expression was achieved by fusing the mature cDNA of mnp1 with the A. oryzae Taka amylase promoter and secretion signal. The 3{prime} untranslated region of the glucoamylase gene of Asperigillus awamori provided the terminator. The recombinant protein (rMnP) was secreted in an active form, permitting rapid detection and purification. Physical and kinetic properties of rMnP were similar to those of the native protein. The A. oryzae expression system is well suited for both mechanistic and site-directed mutagenesis studies. 34 refs., 7 figs., 1 tab.},
doi = {},
journal = {Applied and Environmental Microbiology},
number = 3,
volume = 62,
place = {United States},
year = {Fri Mar 01 00:00:00 EST 1996},
month = {Fri Mar 01 00:00:00 EST 1996}
}