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Title: Radiation reaction of the NAD$sup +$--NADH systems in vitro and in mitochondria as a function of the metabolic stage (in German)

Abstract

Thesis. The methods and results of investigations on the influence of the metabolic state on the effect of 150 kV x rays and /sup 60/Co gamma rays on the system NAD/sup +/--NADH in rat liver mitochondria are described. Irradiation with 2.7 krad already clearly affected the reduction of the pyridine nucleotides in substratedepleted mitochondria with addition of BOH and the respiratory process itself. The development of the radiation damage depends on the incubation conditions after irradiation, with BOH having a protective effect. The genenal redox condition seems to be of importance in this case for the radiation reaction of the mitochondria. The release of NAD from the mitochondria proved to be the principal cause of the inhibition of BOR respiration. Experiments on the radiochemical reactions of NADH in pure water and in solutions with orthophosphate buffer have shown that the phosphate ions or/and the radicals play an important role in the radiation-induced reactions. The presence of cysteine in N/sub 2/-atmosphere enhances the radiation effect on NADH both in buffered and in purely aqueous solutions. The results of these experiments agree well with the hypothesis set up by Bacq and Alexander that says that the physiological condition of the cells ismore » a major factor determining the nadiation reaction of the cells. (GE)« less

Authors:
Publication Date:
Research Org.:
Originating Research Org. not identified
OSTI Identifier:
4332069
NSA Number:
NSA-29-024419
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis. Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: GE (GE)
Country of Publication:
Germany
Language:
German
Subject:
N48110* -Life Sciences-Radiation Effects on Biochemicals- In-Vitro; AQUEOUS SOLUTIONS- BUFFERS- CHEMICAL RADIATION EFFECTS- CYSTEINE- RADIATION CHEMISTRY- RADICALS; GAMMA RADIATION; IN VITRO; IRRADIATION; NADH2; OXYGEN; PHOTON BEAMS; X RADIATION BIOCHEMISTRY- BIOLOGICAL RADIATION EFFECTS- MITOCHONDRIA- REDOX PROCESS- RESPIRATION- SUBSTRATES

Citation Formats

Ojeda-Schwencke, F. Radiation reaction of the NAD$sup +$--NADH systems in vitro and in mitochondria as a function of the metabolic stage. Germany: N. p., 1972. Web.
Ojeda-Schwencke, F. Radiation reaction of the NAD$sup +$--NADH systems in vitro and in mitochondria as a function of the metabolic stage. Germany.
Ojeda-Schwencke, F. Sat . "Radiation reaction of the NAD$sup +$--NADH systems in vitro and in mitochondria as a function of the metabolic stage". Germany.
@article{osti_4332069,
title = {Radiation reaction of the NAD$sup +$--NADH systems in vitro and in mitochondria as a function of the metabolic stage},
author = {Ojeda-Schwencke, F},
abstractNote = {Thesis. The methods and results of investigations on the influence of the metabolic state on the effect of 150 kV x rays and /sup 60/Co gamma rays on the system NAD/sup +/--NADH in rat liver mitochondria are described. Irradiation with 2.7 krad already clearly affected the reduction of the pyridine nucleotides in substratedepleted mitochondria with addition of BOH and the respiratory process itself. The development of the radiation damage depends on the incubation conditions after irradiation, with BOH having a protective effect. The genenal redox condition seems to be of importance in this case for the radiation reaction of the mitochondria. The release of NAD from the mitochondria proved to be the principal cause of the inhibition of BOR respiration. Experiments on the radiochemical reactions of NADH in pure water and in solutions with orthophosphate buffer have shown that the phosphate ions or/and the radicals play an important role in the radiation-induced reactions. The presence of cysteine in N/sub 2/-atmosphere enhances the radiation effect on NADH both in buffered and in purely aqueous solutions. The results of these experiments agree well with the hypothesis set up by Bacq and Alexander that says that the physiological condition of the cells is a major factor determining the nadiation reaction of the cells. (GE)},
doi = {},
journal = {},
number = ,
volume = ,
place = {Germany},
year = {1972},
month = {1}
}

Thesis/Dissertation:
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