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Title: Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae

Abstract

A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation in P steps were inducible. S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol by S. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.

Authors:
; ;  [1]
  1. Osaka Univ. (Japan); and others
Publication Date:
Research Org.:
Oak Ridge National Lab., TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
OSTI Identifier:
431591
Report Number(s):
CONF-900512-
TRN: 96:006510-0029
Resource Type:
Conference
Resource Relation:
Conference: 12. symposium on biotechnology fuels and chemicals, Gatlinburg, TN (United States), 7-11 May 1990; Other Information: PBD: 1991; Related Information: Is Part Of Twelfth symposium on biotechnology for fuels and chemicals; Greenbaum, E. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [Solar Energy Research Inst., Golden, CO (United States)]; PB: 934 p.
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; XYLOSE; METABOLISM; OXIDOREDUCTASES; ENZYME ACTIVITY; BIOTECHNOLOGY; ETHANOL; SACCHAROMYCES CEREVISIAE; DNA SEQUENCING

Citation Formats

Takuma, Shinya, Nakashima, Noriyuki, and Tantirungkij, Manee. Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae. United States: N. p., 1991. Web.
Takuma, Shinya, Nakashima, Noriyuki, & Tantirungkij, Manee. Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae. United States.
Takuma, Shinya, Nakashima, Noriyuki, and Tantirungkij, Manee. Tue . "Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae". United States.
@article{osti_431591,
title = {Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae},
author = {Takuma, Shinya and Nakashima, Noriyuki and Tantirungkij, Manee},
abstractNote = {A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation in P steps were inducible. S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol by S. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1991},
month = {12}
}

Conference:
Other availability
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