Growth kinetics of Bacillus stearothermophilus BR219

Conference · Tue Dec 31 00:00:00 EST 1991

OSTI ID:431585

Worden, R M; Subramanian, R; Bly, M J; Winter, S; Aronson, C L ^[1]

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1. Michigan State Univ., East Lansing, MI (United States)

Bacillus stearothermophilus BR219, a phenol-resistant thermophile, can convert phenol to the specialty chemical catechol. The growth kinetics of this organism were studied in batch, continuous, and immobilized-cell culture. Batch growth was insensitive to pH between 6.0 and 8.0, but little growth occurred at 5.5. In continuous culture on a dilute medium supplemented with 10 mM phenol, several steady states were achieved between dilution rates of 0.25 and 1.3 h{sup -1}. Phenol degradation was found to be uncoupled from growth. Immobilized cells grew rapidly in a rich medium, but cell viability plummeted following a switch to a dilute medium supplemented with 5 mM phenol.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
• OSTI ID: 431585
• Report Number(s): CONF-900512-; TRN: 96:006510-0023
• Resource Relation: Conference: 12. symposium on biotechnology fuels and chemicals, Gatlinburg, TN (United States), 7-11 May 1990; Other Information: PBD: 1991; Related Information: Is Part Of Twelfth symposium on biotechnology for fuels and chemicals; Greenbaum, E. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [Solar Energy Research Inst., Golden, CO (United States)]; PB: 934 p.
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
PHENOL
BIOCONVERSION
BACILLUS
BIOTECHNOLOGY
CHEMICAL FEEDSTOCKS
PYROCATECHOL
BIOCHEMICAL REACTION KINETICS
PH VALUE
CULTURE MEDIA