Effects of pH and anoxia on the cell morphology and radiation sensitivity of escherichia coli
- Univ. of California, Berkeley, CA (United States)
The effects of varying the hydrogen ion concentration and oxygen tension in the environment of Escherichia coli during growth, irradiation, and post-irradiation incubation have been studied. Previous workers had found that when these cells are grown anaerobically, their resistance to x rays is greatly increased. This resistance was attributed to the production of an anoxic condition within the cell during growth, in the same way as and additive to resistance conferred by anoxic conditions during irradiation. It is shown here that protection conferred by anaerobic growth is independent of anoxia during irradiation, and can be abolished by causing growth to occur in alkaline rather than the usual acid conditions. The resistance resulting from anaerobic acid growth is attributed to the multinucleate nature of the cells, as shown by cytological observations, DNA analysis, and the shape of survival curves.
- Research Organization:
- Univ. of California, Berkeley, CA (United States)
- Sponsoring Organization:
- US Atomic Energy Commission (AEC)
- DOE Contract Number:
- W-7405-ENG-48
- NSA Number:
- NSA-13-011539
- OSTI ID:
- 4276926
- Report Number(s):
- UCRL-8668
- Resource Relation:
- Other Information: Orig. Receipt Date: 31-DEC-59
- Country of Publication:
- United States
- Language:
- English
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