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Title: Photosynthetic acclimation to elevated CO{sub 2} occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content

Abstract

Inhibition of net carbon assimilation rates during growth at elevated CO{sub 2} was studied in transgenic tobacco (Nicotiana tabacum L.) plants containing zero to two copies of antisense DNA sequences to the small subunit polypeptide (rbcS) gene of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). High- and low-Rubisco tobacco plants were obtained from the selfed progeny of the original line 3 transformant. Assimilation rates of high- and low-Rubisco tobacco plants increased 22 and 71%, respectively, when transferred from 35- to 70-Pa CO{sub 2} chamber air at 900 {mu}mol m{sup -2} s{sup -1} photon flux density. However, CO{sub 2}-dependent increases of net carbon assimilation rates of high- and low-Rubisco plants virtually disappeared after 9 d of growth in elevated CO{sub 2} chamber air. Total above-ground dry matter production of high- and low-Rubisco plants was 28 and 53% greater, respectively, after 9 d of growth at 70 Pa compared with 35 Pa CO{sub 2}. Most of this dry weight gain was due to increased specific leaf weight. Rubisco activity, Rubisco protein, and total chlorophyll were lower in both high- and low-Rubisco plants grown in enriched compared with ambient CO{sub 2} chamber air. Soluble leaf protein also decreased in response to CO{sub 2} enrichment in high- butmore » not in low-Rub tobacco plants. Decreased Rubisco activities in CO{sub 2}adapted high- and low-Rubisco plants were not attributable to changes in activation state of the enzyme. Carbonic anhydrase activities and subunit levels measured with specific antibodies were similar in high- and low-Rubisco tobacco plants and were unchanged by CO{sub 2} enrichment. Collectively, these findings suggested that photosynthetic acclimation to enriched CO{sub 2} occurred in tobacco plants either with or without transgenically decreased Rubisco in CO{sub 2}-adapted tobacco plants was related to decreased specific activity of this enzyme. 35 refs., 2 figs., 1 tab.« less

Authors:
; ;  [1]
  1. Iowa State Univ., Ames, IA (United States)
Publication Date:
OSTI Identifier:
426053
Resource Type:
Journal Article
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 104; Journal Issue: 2; Other Information: PBD: Feb 1994
Country of Publication:
United States
Language:
English
Subject:
56 BIOLOGY AND MEDICINE, APPLIED STUDIES; CARBON DIOXIDE; BIOLOGICAL EFFECTS; BIOLOGICAL ADAPTATION; TOBACCO; PHOTOSYNTHESIS; ENZYMES; ECOLOGICAL CONCENTRATION

Citation Formats

Sicher, R C, Kremer, D F, and Rodermel, S R. Photosynthetic acclimation to elevated CO{sub 2} occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content. United States: N. p., 1994. Web.
Sicher, R C, Kremer, D F, & Rodermel, S R. Photosynthetic acclimation to elevated CO{sub 2} occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content. United States.
Sicher, R C, Kremer, D F, and Rodermel, S R. Tue . "Photosynthetic acclimation to elevated CO{sub 2} occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content". United States.
@article{osti_426053,
title = {Photosynthetic acclimation to elevated CO{sub 2} occurs in transformed tobacco with decreased ribulose-1,5-bisphosphate carboxylase/oxygenase content},
author = {Sicher, R C and Kremer, D F and Rodermel, S R},
abstractNote = {Inhibition of net carbon assimilation rates during growth at elevated CO{sub 2} was studied in transgenic tobacco (Nicotiana tabacum L.) plants containing zero to two copies of antisense DNA sequences to the small subunit polypeptide (rbcS) gene of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). High- and low-Rubisco tobacco plants were obtained from the selfed progeny of the original line 3 transformant. Assimilation rates of high- and low-Rubisco tobacco plants increased 22 and 71%, respectively, when transferred from 35- to 70-Pa CO{sub 2} chamber air at 900 {mu}mol m{sup -2} s{sup -1} photon flux density. However, CO{sub 2}-dependent increases of net carbon assimilation rates of high- and low-Rubisco plants virtually disappeared after 9 d of growth in elevated CO{sub 2} chamber air. Total above-ground dry matter production of high- and low-Rubisco plants was 28 and 53% greater, respectively, after 9 d of growth at 70 Pa compared with 35 Pa CO{sub 2}. Most of this dry weight gain was due to increased specific leaf weight. Rubisco activity, Rubisco protein, and total chlorophyll were lower in both high- and low-Rubisco plants grown in enriched compared with ambient CO{sub 2} chamber air. Soluble leaf protein also decreased in response to CO{sub 2} enrichment in high- but not in low-Rub tobacco plants. Decreased Rubisco activities in CO{sub 2}adapted high- and low-Rubisco plants were not attributable to changes in activation state of the enzyme. Carbonic anhydrase activities and subunit levels measured with specific antibodies were similar in high- and low-Rubisco tobacco plants and were unchanged by CO{sub 2} enrichment. Collectively, these findings suggested that photosynthetic acclimation to enriched CO{sub 2} occurred in tobacco plants either with or without transgenically decreased Rubisco in CO{sub 2}-adapted tobacco plants was related to decreased specific activity of this enzyme. 35 refs., 2 figs., 1 tab.},
doi = {},
url = {https://www.osti.gov/biblio/426053}, journal = {Plant Physiology (Bethesda)},
number = 2,
volume = 104,
place = {United States},
year = {1994},
month = {2}
}