Degradation of DNA in Haemophilus influenzae cells after x-ray irradiation. I. Experimental results

Journal Article · Thu May 01 00:00:00 EDT 1975 · Biophys. J., v. 15, no. 5, pp. 441-453

OSTI ID:4158354

Randolph, M L; Setlow, J K

Sequential measurements of DNA in Haemophilus influenzae cells after x- ray irradiation show rapid initial degradation of DNA followed by a plateau after about 40 min at normal growth conditions. Both the initial rate and final amount of degradation increase with radiation exposure. Degradation is somewhat greater in stationary-phase than in log-phase cells, but colony-forming ability (CFA) is independent of cell stage. Distributions of single-strand lengths of DNA in unirradiated or irradiated cases, as measured by alkaline sucrose gradient techniques, are neither monodispersive nor random, and possible causes for nonrandomness are discussed. (auth)

• Research Organization: Oak Ridge National Lab., TN
• NSA Number: NSA-33-007424
• OSTI ID: 4158354
• Journal Information: Biophys. J., v. 15, no. 5, pp. 441-453, Other Information: Orig. Receipt Date: 30-JUN-76
• Country of Publication: United States
• Language: English

Related Subjects

N48120* -Life Sciences-Radiation Effects on Biochemicals- In Microorganisms
N48310 -Life Sciences-Radiation Effects on Microorganisms-Basic Studies
560112* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Biochemicals-In Microorganisms
*DNA- STRAND BREAKS
*HAEMOPHILUS- BIOLOGICAL RADIATION EFFECTS
*STRAND BREAKS- RADIOINDUCTION
DOSE-RESPONSE RELATIONSHIPS
IRRADIATION
X RADIATION