Endonuclease III: an endonuclease from Escherichia coli that introduces single polynucleotide chain scissions in ultraviolet-irradiated DNA
- ed.
An endonuclease that makes single polynucleotide chain scissions in uv- irradiated DNA has been purified from Escherichia coli. The activity has the following properties: (1) unirradiated DNA is attacked very little if at all; single-stranded DNA is not attacked, whether irradiated or not; there is no requirement for divalent cations, and the activity is not affected by the addition of EDTA; the pH optimum is approximately 7; the activity is inhibited by 1 M NaCl, single-stranded DNA, transfer RNA, and unirradiated double-stranded DNA; the sedimentation coefficient, S$sub 20$,/sub W'/ is approximately 2.6; it is a basic protein. The enzyme is tentatively named E. coli endonuclease III. The physiological function of the endonuclease has not yet been established. (auth)
- Research Organization:
- Harvard Univ., Cambridge, MA
- NSA Number:
- NSA-33-012267
- OSTI ID:
- 4144843
- Resource Relation:
- Conference: DNA repair processes conference, Squaw Valley, California, USA, Feb 1974; Other Information: See CONF-740231--PA. Orig. Receipt Date: 30-JUN-76; Related Information: Molecular mechanisms for repair of DNA. Part A
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
N48110 -Life Sciences-Radiation Effects on Biochemicals-In-Vitro
560131* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies
*DNA- BIOLOGICAL RADIATION EFFECTS
*EDTA- BIOLOGICAL EFFECTS
*ESCHERICHIA COLI- BIOLOGICAL RADIATION EFFECTS
*NUCLEASES- METABOLISM
*PH VALUE- BIOLOGICAL EFFECTS
*SODIUM CHLORIDES- BIOLOGICAL EFFECTS
CATALYSIS
NUCLEOTIDES
RNA
STRAND BREAKS
ULTRAVIOLET RADIATION