Altered ganglioside biosynthesis in mouse cell cultures following transformation with chemical carcinogens and x-irradiation
Chemically and x-ray-transformed subclones of BALB/c 3T3 mouse embryo cells were found to have reduced amounts of the mono- and disialogangliosides galactosyl-N-acetylgalactosaminyl-[N-acetylneuraminyl]-galactosylglucosylceramide (G/sub M$sup 1$/) and N-acetylneuraminylgalactosyl-N-acetylgalactosaminyl-[N- acetylneuraminyl]-galactosylglucosylceramide (G/sub D$sup 1$a/), and increased amounts of N-acetylgalactosaminyl-[N-acetylneuraminyl]-galactosylglucosylceramide (G/sub M$sup 2$/). The activity of the enzyme UDP-Gal:G/sub M$sup 2$/ galactosyltransferase was reduced to between 2.7 and 14.3 percent of normal in the transformed clones. Other ganglioside glycosyltransferase activities were unaffected. This enzymatic change was consistent with the observed alteration in ganglioside pattern in the transformed cells. The residual galactosyltransferase activity in the transformed cells was kinetically similar to the normal enzyme, suggesting that transformation alters ganglioside biosynthesis by blocking enzyme synthesis at the translational or transcriptional levels. (auth)
- Research Organization:
- National Inst. of Health, Bethesda, MD
- NSA Number:
- NSA-33-020485
- OSTI ID:
- 4083048
- Journal Information:
- J. Biol. Chem., v. 250, no. 1, pp. 55-60, Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
Similar Records
Regulation of chondroitin sulfate synthesis: effect of. beta. -xylosides on synthesis of chondroitin sulfate proteoglycan, chondroitin sulfate chains, and core protein
Labeling of the retina and optic tectum gangliosides and glycoproteins of chickens in darkness or exposed to light
Related Subjects
N46110 -Instrumentation-Radiation Detection Instruments-General Detectors & Monitors
560121* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Cells-External Source
*CARCINOGENS- BIOLOGICAL EFFECTS
*EMBRYONIC CELLS- BIOLOGICAL RADIATION EFFECTS
*GANGLIOSIDES- BIOSYNTHESIS
*TRANSFERASES- METABOLISM
CATALYSIS
CELL CULTURES
MICE
X RADIATION