skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: STUDIES ON THE INDUCTION OF MUTATIONS BY Co$sup 60$ IRRADIATION OF EYE SPROUTS OF THE POTATO (in German)

Abstract

Sprouting, illuminated potato shoots of the Sieglinde variety potato were exposed to gamma rays at doses of 4000, 6000, and 8000 r referred to as series I, II, and III. Nodal cuttings were prepared from the longest shoot of 40 tubers of each series by cutting the shoots through beneath the nodes (basipetal) and growing separately until they became rooted plants. The shorter shoots were left intact and used for making eye cuttings. After storage of the tubers for 37 days at 12 to 15 deg C with illumination, nodal cuttings were prepared and grown on a further 40 tubers of series II and III (IIa and IIIa). The nodal cuttings of series I, II, and III showed abnormalities in the vM/sub 1/ generation, which were expressed as stunted growth, swelling of the nodes, misshapen leaves, formation of aerial tubers and aerial stolons with or without tubers. These abnormalities were both qualitatively and quantitatively essentially independent of the dosage used. The eye cuttings of the same series showed these abnormalities only to a very limited extent. No abnormalities were found among nodal cuttings of series IIa and IIIa grown under identical conditions. The same was true for cuttings coming frommore » severely altered plants of series III (apical cuttings). The growth abnormalities of the nodal cuttings in the three series are considered to be the result of disturbance to the plant hormone system (especially auxins) and are discussed in relation to this system. The lack of abnormalities in the eye cuttings of series I, II, and III is thought to be caused by the damage in entire shoots being more quickly brought into equilibrium again. In the cuttings of series IIa and IIIa, recuperation after growth-hormone damage may have taken place during the 37-day continuous storage period. In vM/ sub 2/, 42 anomalous plants were identified. The changes involved only leaves, stems, and chlorophyll formation. Changes in other characters could not be observed because of a serious attack by leaf-roll virus and scab. The considerable vegetative division of the series of nodal cuttings did not lead to a higher yield of abnormal plants compared with the eye cuttings. The vM/sub 3/ generation was limited to a single tuber from each plant. Morphologic observation in this generation was hindered by invariable attack by leaf-roll virus. Several plants were observed, however, which scarcely showed any symptoms of this disease. Plants in the vM/sub 4/ generation from five tubers each of the vM/sub 3/ plants showing almost no symptoms were investigated for phloem necrosis. A few clones were recorded as not attacked or only very slightly attacked, The mutant plants in the vM/sub 2/ generation showed some somatic segregation in vM/ sub 3/, indicating a marked chimeric nature for these individuals. (BBB)« less

Authors:
;
Publication Date:
Research Org.:
Max-Planck-Institut fur Zuechtungsforschung, Cologne
OSTI Identifier:
4056188
NSA Number:
NSA-18-017616
Resource Type:
Journal Article
Journal Name:
Zeitschrift fuer Pflanzenzuechtung (West Germany)
Additional Journal Information:
Journal Volume: Vol: 49; Other Information: Orig. Receipt Date: 31-DEC-64
Country of Publication:
Country unknown/Code not available
Language:
German
Subject:
BIOLOGY AND MEDICINE; AGRICULTURE; AUXINS; CHLOROPHYLL; COBALT 60; DISEASES; GAMMA RADIATION; HORMONES; IRRADIATION; LIGHT; MALFORMATIONS; METABOLISM; MUTATIONS; ORGANIC ACIDS; PERFORMANCE; PIGMENTS; PLANT CELLS; PLANTS; POTATOES; RADIATION DOSES; RADIATION EFFECTS; RADIOSENSITIVITY; REPRODUCTION; SEEDS; TEMPERATURE; TESTING; TISSUES; VARIATIONS; VIRUSES

Citation Formats

Rudorf, W, and Woehrmann, K. STUDIES ON THE INDUCTION OF MUTATIONS BY Co$sup 60$ IRRADIATION OF EYE SPROUTS OF THE POTATO. Country unknown/Code not available: N. p., 1963. Web.
Rudorf, W, & Woehrmann, K. STUDIES ON THE INDUCTION OF MUTATIONS BY Co$sup 60$ IRRADIATION OF EYE SPROUTS OF THE POTATO. Country unknown/Code not available.
Rudorf, W, and Woehrmann, K. 1963. "STUDIES ON THE INDUCTION OF MUTATIONS BY Co$sup 60$ IRRADIATION OF EYE SPROUTS OF THE POTATO". Country unknown/Code not available.
@article{osti_4056188,
title = {STUDIES ON THE INDUCTION OF MUTATIONS BY Co$sup 60$ IRRADIATION OF EYE SPROUTS OF THE POTATO},
author = {Rudorf, W and Woehrmann, K},
abstractNote = {Sprouting, illuminated potato shoots of the Sieglinde variety potato were exposed to gamma rays at doses of 4000, 6000, and 8000 r referred to as series I, II, and III. Nodal cuttings were prepared from the longest shoot of 40 tubers of each series by cutting the shoots through beneath the nodes (basipetal) and growing separately until they became rooted plants. The shorter shoots were left intact and used for making eye cuttings. After storage of the tubers for 37 days at 12 to 15 deg C with illumination, nodal cuttings were prepared and grown on a further 40 tubers of series II and III (IIa and IIIa). The nodal cuttings of series I, II, and III showed abnormalities in the vM/sub 1/ generation, which were expressed as stunted growth, swelling of the nodes, misshapen leaves, formation of aerial tubers and aerial stolons with or without tubers. These abnormalities were both qualitatively and quantitatively essentially independent of the dosage used. The eye cuttings of the same series showed these abnormalities only to a very limited extent. No abnormalities were found among nodal cuttings of series IIa and IIIa grown under identical conditions. The same was true for cuttings coming from severely altered plants of series III (apical cuttings). The growth abnormalities of the nodal cuttings in the three series are considered to be the result of disturbance to the plant hormone system (especially auxins) and are discussed in relation to this system. The lack of abnormalities in the eye cuttings of series I, II, and III is thought to be caused by the damage in entire shoots being more quickly brought into equilibrium again. In the cuttings of series IIa and IIIa, recuperation after growth-hormone damage may have taken place during the 37-day continuous storage period. In vM/ sub 2/, 42 anomalous plants were identified. The changes involved only leaves, stems, and chlorophyll formation. Changes in other characters could not be observed because of a serious attack by leaf-roll virus and scab. The considerable vegetative division of the series of nodal cuttings did not lead to a higher yield of abnormal plants compared with the eye cuttings. The vM/sub 3/ generation was limited to a single tuber from each plant. Morphologic observation in this generation was hindered by invariable attack by leaf-roll virus. Several plants were observed, however, which scarcely showed any symptoms of this disease. Plants in the vM/sub 4/ generation from five tubers each of the vM/sub 3/ plants showing almost no symptoms were investigated for phloem necrosis. A few clones were recorded as not attacked or only very slightly attacked, The mutant plants in the vM/sub 2/ generation showed some somatic segregation in vM/ sub 3/, indicating a marked chimeric nature for these individuals. (BBB)},
doi = {},
url = {https://www.osti.gov/biblio/4056188}, journal = {Zeitschrift fuer Pflanzenzuechtung (West Germany)},
number = ,
volume = Vol: 49,
place = {Country unknown/Code not available},
year = {1963},
month = {8}
}