skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes

Abstract

We have demonstrated, for the first time, that capillary electrophoresis (CE) can be interfaced with low-temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization. Detection by laser-induced fluorescence spectroscopy, under fluorescence non-line-narrowing and line-narrowing conditions, provides three-dimensional electropherograms and FLN spectra, which lead to significantly improved overall resolution and allow for structural characterization (`fingerprinting`) of molecular analytes. This novel CE-FLN system consists of a modular CE system, instrumentation for FLN spectroscopy, and a specially designed capillary cryostat (CC). An absorbance detector serves to determine the migration rates of analytes. After the 77 K fluorescence-based electropherogram is generated, the temperature of the capillary is lowered to 4.2 K for high-resolution FLN characterization. Automated translation of the CC and capillary in the direction of the capillary axis allows the separated analytes to be sequentially characterized by fluorescence spectroscopy as the capillary is translated through the laser excitation region. Detection of fluorescence from stationary CE-separated analytes significantly improves the accuracy of quantitation and structural characterization. 41 refs., 4 figs.

Authors:
; ; ;  [1]
  1. Ames Lab., IA (United States)
Publication Date:
OSTI Identifier:
405338
DOE Contract Number:  
W-7405-ENG-82
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington)
Additional Journal Information:
Journal Volume: 68; Journal Issue: 15; Other Information: PBD: 1 Aug 1996
Country of Publication:
United States
Language:
English
Subject:
40 CHEMISTRY; AROMATICS; STRUCTURAL CHEMICAL ANALYSIS; EMISSION SPECTRA; HYDROCARBONS; CAPILLARY FLOW; ELECTROPHORESIS; FLUORESCENCE; LASER RADIATION

Citation Formats

Jankowiak, R, Zamzow, D, Ding, W, Small, G J, and Iowa State Univ., Ames, IA. Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes. United States: N. p., 1996. Web. doi:10.1021/ac960251j.
Jankowiak, R, Zamzow, D, Ding, W, Small, G J, & Iowa State Univ., Ames, IA. Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes. United States. doi:10.1021/ac960251j.
Jankowiak, R, Zamzow, D, Ding, W, Small, G J, and Iowa State Univ., Ames, IA. Thu . "Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes". United States. doi:10.1021/ac960251j.
@article{osti_405338,
title = {Capillary electrophoresis-fluorescence line-narrowing system for on-line structural characterization of molecular analytes},
author = {Jankowiak, R and Zamzow, D and Ding, W and Small, G J and Iowa State Univ., Ames, IA},
abstractNote = {We have demonstrated, for the first time, that capillary electrophoresis (CE) can be interfaced with low-temperature fluorescence line-narrowing (FLN) spectroscopy for on-line structural characterization. Detection by laser-induced fluorescence spectroscopy, under fluorescence non-line-narrowing and line-narrowing conditions, provides three-dimensional electropherograms and FLN spectra, which lead to significantly improved overall resolution and allow for structural characterization (`fingerprinting`) of molecular analytes. This novel CE-FLN system consists of a modular CE system, instrumentation for FLN spectroscopy, and a specially designed capillary cryostat (CC). An absorbance detector serves to determine the migration rates of analytes. After the 77 K fluorescence-based electropherogram is generated, the temperature of the capillary is lowered to 4.2 K for high-resolution FLN characterization. Automated translation of the CC and capillary in the direction of the capillary axis allows the separated analytes to be sequentially characterized by fluorescence spectroscopy as the capillary is translated through the laser excitation region. Detection of fluorescence from stationary CE-separated analytes significantly improves the accuracy of quantitation and structural characterization. 41 refs., 4 figs.},
doi = {10.1021/ac960251j},
journal = {Analytical Chemistry (Washington)},
number = 15,
volume = 68,
place = {United States},
year = {1996},
month = {8}
}