TRANSPLANTATION OF PERITONEAL CELLS
Serological methods were used to establish the strain of origin of erythrocytes and leukocytes in mice given a lethal dose of irradiation and a single injection of peritoneal cells. F/sub 1/ hybrid mice were used as donors, and cells were obtained by washing the peritoneal cavity with heparinized saline. The usual yield was 3 to 5 million cells per young adult donor. In all experiments /sup 59/Fe uptake was measured within the second week after transplantation. When appreciable uptake was found, reaction of the cells with donor-specific antisera released the label into the lysate, whereas reaction with nonspecific antiserum or nonimmune sera released none of the label. Histologic studies were made on spleen, bone marrow and lymph nodes obtained at autopsy from transplanted, as well as control, irradiated mice. Transplanted mice showed microscopic evidence of erythropoiesis and granulopoiesis in spleen red pulp and bone marrow. Lymphatic tissue proliferation was noted in spleen white pulp and cortex of lymph nodes. Thus, stem cells of the hemopoietic and lymphatic systems are normally present in the peritoneal cavity as well as in bone marrow, spleen, and circulating blood of mice. (auth)
- Research Organization:
- Oak Ridge National Lab., Tenn.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-18-019591
- OSTI ID:
- 4024923
- Journal Information:
- Annals of the New York Academy of Sciences (U.S.), Vol. Vol: 114; Other Information: Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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