UPTAKE OF RADIONUCLIDES BY MICROORGANISMS. EMPHASIS UPON THE MECHANISM OF UPTAKE OF CALCIUM IN MARINE BACTERIA COMPARED WITH THE MOLLUSC, STROMBUS GIGAS. Final Report, April 15, 1963 to December 14, 1963
The source of carbonate in marine molluscan shell formation was investigated in the immature queen conch, 8trombus gigas, using Na/sup 14/CO/sub 3/ as a source of inorganic carbonate and /sup 14/C-L-alanine, L-serine, and L - glutamic acid as organic sources. The uptake of the labeled compounds, and of / sup 45/Ca as CaCl/sub 2/, was determined as related to the physical activity of the animal. Results indicate that the conch can utilize many substances dissolved in the sea water environment as a source of carbonate and that the site of absorption is not limited to the gut wall. The role of marine bacterin in biological calcification in the sea was also investigated. Results are reported from studies of surface binding of Ca and Mg by the cell envelope of Pseudomonas piscicida, a bacteria found in shallow water marine sediments. Calcium-45 and / sup 14/C-alanine were used as tracers. It was concluded that at least two mechanisms are directly involved in the uptake of Ca and Mg by P. piscicida from sea water and that as the cells age increased amounts of CaCO/sub 3/ are deposited but MgCO/sub 3/ deposition remains relatively static. (C.H.)
- Research Organization:
- Miami, Fla. Univ. Marine Lab.
- DOE Contract Number:
- AT(40-1)-2855
- NSA Number:
- NSA-18-019520
- OSTI ID:
- 4020528
- Report Number(s):
- TID-20499
- Resource Relation:
- Other Information: Orig. Receipt Date: 31-DEC-64
- Country of Publication:
- United States
- Language:
- English
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