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Title: Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3

Abstract

To enhance the cytogenetic expression of the fragile X chromosome, we studied the effects of hyperoxia and caffeine on the induction of fragile Xq27.3. A lymphoblastoid cell line (GM 06912) derived from a fragile X male proband was cultured in RPMI 1640 containing 16% dialyzed fetal calf serum. The cells were synchronously subjected to one of 3 different atmospheric oxygen tensions (21%, 21.3 kPa, hyperoxic) during the last 24 hours of the 72 hour culture, immediately after the addition of 2{prime}-deoxy-5-fluorouridine (FUdR) at 25 ng/ml. To study the enhancing effect of caffeine, with or without hyperoxia, a second set of cultures was additionally subjected to caffeine (2.5 mM) during the last 6 hours of the culture. When the fragility of hyperoxic cells (38.1 kPa dissolved oxygen) was compared to that of normoxic control cells (13.3 kPa dissolved oxygen), the difference was significant (P < 0.05). These data suggest that there is a mean increase in the fragile Xq27.3 expressivity as the dissolved oxygen tension increases. Additionally, we observed that caffeine, with or without hyperoxia, significantly (P <0.05) suppressed the expression of the fragile X site in this lymphoblastoid cell line. 34 refs., 2 tabs.

Authors:
; ;  [1]
  1. Armed Forces Institute of Pathology, Washington, DC (United States); and others
Publication Date:
OSTI Identifier:
401800
Resource Type:
Journal Article
Journal Name:
American Journal of Medical Genetics
Additional Journal Information:
Journal Volume: 61; Journal Issue: 4; Other Information: PBD: 2 Feb 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; HUMAN X CHROMOSOME; CHROMOSOMAL ABERRATIONS; SISTER CHROMATID EXCHANGES; GENETIC EFFECTS; ANIMAL CELLS; CELL PROLIFERATION; CAFFEINE; STATISTICS; HEREDITARY DISEASES; MENTAL DISORDERS

Citation Formats

Rafi, S K, Surana, R B, and Christopher, K L. Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3. United States: N. p., 1996. Web. doi:10.1002/(SICI)1096-8628(19960202)61:4<299::AID-AJMG1>3.0.CO;2-Q.
Rafi, S K, Surana, R B, & Christopher, K L. Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3. United States. https://doi.org/10.1002/(SICI)1096-8628(19960202)61:4<299::AID-AJMG1>3.0.CO;2-Q
Rafi, S K, Surana, R B, and Christopher, K L. 1996. "Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3". United States. https://doi.org/10.1002/(SICI)1096-8628(19960202)61:4<299::AID-AJMG1>3.0.CO;2-Q.
@article{osti_401800,
title = {Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3},
author = {Rafi, S K and Surana, R B and Christopher, K L},
abstractNote = {To enhance the cytogenetic expression of the fragile X chromosome, we studied the effects of hyperoxia and caffeine on the induction of fragile Xq27.3. A lymphoblastoid cell line (GM 06912) derived from a fragile X male proband was cultured in RPMI 1640 containing 16% dialyzed fetal calf serum. The cells were synchronously subjected to one of 3 different atmospheric oxygen tensions (21%, 21.3 kPa, hyperoxic) during the last 24 hours of the 72 hour culture, immediately after the addition of 2{prime}-deoxy-5-fluorouridine (FUdR) at 25 ng/ml. To study the enhancing effect of caffeine, with or without hyperoxia, a second set of cultures was additionally subjected to caffeine (2.5 mM) during the last 6 hours of the culture. When the fragility of hyperoxic cells (38.1 kPa dissolved oxygen) was compared to that of normoxic control cells (13.3 kPa dissolved oxygen), the difference was significant (P < 0.05). These data suggest that there is a mean increase in the fragile Xq27.3 expressivity as the dissolved oxygen tension increases. Additionally, we observed that caffeine, with or without hyperoxia, significantly (P <0.05) suppressed the expression of the fragile X site in this lymphoblastoid cell line. 34 refs., 2 tabs.},
doi = {10.1002/(SICI)1096-8628(19960202)61:4<299::AID-AJMG1>3.0.CO;2-Q},
url = {https://www.osti.gov/biblio/401800}, journal = {American Journal of Medical Genetics},
number = 4,
volume = 61,
place = {United States},
year = {Fri Feb 02 00:00:00 EST 1996},
month = {Fri Feb 02 00:00:00 EST 1996}
}