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Title: Kinetics of chromate reduction during naphthalene degradation in a mixed culture

Abstract

A mixed culture of Bacillus sp. K1 and Sphingomonas paucimobilis EPA 505 was exposed to chromate and naphthalene. Batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. Chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. Chromate reduction decreased in the mixed culture when a lower ratio of S. paucimobilis EPA 505 to Bacillus sp. K1 was utilized. A kinetic model incorporating a term for the cell density ratio is proposed to describe chromate reduction in the mixed culture under both chromate limited and electron donor limited conditions. The validity of the model, and its parameter values, was verified by experimental data generated under a variety of initial population compositions and a broad range of chromate concentrations. The consistent result of experimental data with model predictions implies that the model is useful for evaluating the interactions and the use of mixed culture for chromate removal.

Authors:
;  [1];  [2]
  1. EPA National Risk Management Research Lab., Ada, OK (United States). Subsurface Protection and Remediation Div.
  2. EPA National Health and Environment Exposure Research Lab., Gulf Breeze, FL (United States). Gulf Ecology Div.
Publication Date:
OSTI Identifier:
398162
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering
Additional Journal Information:
Journal Volume: 52; Journal Issue: 3; Other Information: PBD: 5 Nov 1996
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; CHROMIUM; BIODEGRADATION; NAPHTHALENE; BACTERIA; BATCH CULTURE; REACTION KINETICS; REMEDIAL ACTION; MATHEMATICAL MODELS

Citation Formats

Shen, H., Sewell, G.W., and Pritchard, P.H.. Kinetics of chromate reduction during naphthalene degradation in a mixed culture. United States: N. p., 1996. Web. doi:10.1002/(SICI)1097-0290(19961105)52:3<357::AID-BIT1>3.0.CO;2-J.
Shen, H., Sewell, G.W., & Pritchard, P.H.. Kinetics of chromate reduction during naphthalene degradation in a mixed culture. United States. doi:10.1002/(SICI)1097-0290(19961105)52:3<357::AID-BIT1>3.0.CO;2-J.
Shen, H., Sewell, G.W., and Pritchard, P.H.. Tue . "Kinetics of chromate reduction during naphthalene degradation in a mixed culture". United States. doi:10.1002/(SICI)1097-0290(19961105)52:3<357::AID-BIT1>3.0.CO;2-J.
@article{osti_398162,
title = {Kinetics of chromate reduction during naphthalene degradation in a mixed culture},
author = {Shen, H. and Sewell, G.W. and Pritchard, P.H.},
abstractNote = {A mixed culture of Bacillus sp. K1 and Sphingomonas paucimobilis EPA 505 was exposed to chromate and naphthalene. Batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. Chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. Chromate reduction decreased in the mixed culture when a lower ratio of S. paucimobilis EPA 505 to Bacillus sp. K1 was utilized. A kinetic model incorporating a term for the cell density ratio is proposed to describe chromate reduction in the mixed culture under both chromate limited and electron donor limited conditions. The validity of the model, and its parameter values, was verified by experimental data generated under a variety of initial population compositions and a broad range of chromate concentrations. The consistent result of experimental data with model predictions implies that the model is useful for evaluating the interactions and the use of mixed culture for chromate removal.},
doi = {10.1002/(SICI)1097-0290(19961105)52:3<357::AID-BIT1>3.0.CO;2-J},
journal = {Biotechnology and Bioengineering},
number = 3,
volume = 52,
place = {United States},
year = {1996},
month = {11}
}