Kinetics of chromate reduction during naphthalene degradation in a mixed culture
- EPA National Risk Management Research Lab., Ada, OK (United States). Subsurface Protection and Remediation Div.
- EPA National Health and Environment Exposure Research Lab., Gulf Breeze, FL (United States). Gulf Ecology Div.
A mixed culture of Bacillus sp. K1 and Sphingomonas paucimobilis EPA 505 was exposed to chromate and naphthalene. Batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. Chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. Chromate reduction decreased in the mixed culture when a lower ratio of S. paucimobilis EPA 505 to Bacillus sp. K1 was utilized. A kinetic model incorporating a term for the cell density ratio is proposed to describe chromate reduction in the mixed culture under both chromate limited and electron donor limited conditions. The validity of the model, and its parameter values, was verified by experimental data generated under a variety of initial population compositions and a broad range of chromate concentrations. The consistent result of experimental data with model predictions implies that the model is useful for evaluating the interactions and the use of mixed culture for chromate removal.
- OSTI ID:
- 398162
- Journal Information:
- Biotechnology and Bioengineering, Vol. 52, Issue 3; Other Information: PBD: 5 Nov 1996
- Country of Publication:
- United States
- Language:
- English
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